Rapid assessment of different oxygenic phototrophs and single-cell photosynthesis with multicolour variable chlorophyll fluorescence imaging
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Rapid assessment of different oxygenic phototrophs and single-cell photosynthesis with multicolour variable chlorophyll fluorescence imaging. / Trampe, Erik Christian Løvbjerg; Kolbowski, J.; Schreiber, U.; Kuhl, M.
In: Marine Biology, Vol. 158, No. 7, 2011, p. 1667-1675.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Rapid assessment of different oxygenic phototrophs and single-cell photosynthesis with multicolour variable chlorophyll fluorescence imaging
AU - Trampe, Erik Christian Løvbjerg
AU - Kolbowski, J.
AU - Schreiber, U.
AU - Kuhl, M.
PY - 2011
Y1 - 2011
N2 - We present a new system for microscopic multicolour variable chlorophyll fluorescence imaging of aquatic phototrophs. The system is compact and portable and enables microscopic imaging of photosynthetic performance of individual cells and chloroplasts using different combinations of blue, green, red or white light. Automated sequential exposure of microscopic samples to the three excitation colours enables subsequent deconvolution of the resulting fluorescence signals and colour marking of cells with different photopigmentation, i.e., cyanobacteria, green algae, red algae and diatoms. The photosynthetic activity in complex mixtures of phototrophs and natural samples can thus be assigned to different types of phototrophs, which can be quantified simultaneously. Here, we describe the composition and performance of the new imaging system and present applications with both natural phytoplankton and microalgal culture samples
AB - We present a new system for microscopic multicolour variable chlorophyll fluorescence imaging of aquatic phototrophs. The system is compact and portable and enables microscopic imaging of photosynthetic performance of individual cells and chloroplasts using different combinations of blue, green, red or white light. Automated sequential exposure of microscopic samples to the three excitation colours enables subsequent deconvolution of the resulting fluorescence signals and colour marking of cells with different photopigmentation, i.e., cyanobacteria, green algae, red algae and diatoms. The photosynthetic activity in complex mixtures of phototrophs and natural samples can thus be assigned to different types of phototrophs, which can be quantified simultaneously. Here, we describe the composition and performance of the new imaging system and present applications with both natural phytoplankton and microalgal culture samples
KW - FAST REPETITION RATE
KW - INDIVIDUAL ALGAL CELLS
KW - A FLUORESCENCE
KW - IN-VIVO
KW - MICROBIAL COMMUNITIES
KW - MARINE-PHYTOPLANKTON
KW - LIGHT CURVES
KW - PERFORMANCE
KW - FLUOROMETRY
KW - SEA
U2 - 10.1007/s00227-011-1663-1
DO - 10.1007/s00227-011-1663-1
M3 - Journal article
VL - 158
SP - 1667
EP - 1675
JO - Marine Biology
JF - Marine Biology
SN - 0025-3162
IS - 7
ER -
ID: 37368127