Phagotrophic Protists Modulate Copper Resistance of the Bacterial Community in Soil

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Phagotrophic Protists Modulate Copper Resistance of the Bacterial Community in Soil. / Lv, Zhenguang; Xu, Min; Liu, Ying; Rønn, Regin; Rensing, Christopher; Liu, Song; Gao, Shenghan; Liao, Hao; Liu, Yu Rong; Chen, Wenli; Zhu, Yong Guan; Huang, Qiaoyun; Hao, Xiuli.

In: Environmental Science and Technology, Vol. 57, No. 9, 2023, p. 3590-3601.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Lv, Z, Xu, M, Liu, Y, Rønn, R, Rensing, C, Liu, S, Gao, S, Liao, H, Liu, YR, Chen, W, Zhu, YG, Huang, Q & Hao, X 2023, 'Phagotrophic Protists Modulate Copper Resistance of the Bacterial Community in Soil', Environmental Science and Technology, vol. 57, no. 9, pp. 3590-3601. https://doi.org/10.1021/acs.est.2c07136

APA

Lv, Z., Xu, M., Liu, Y., Rønn, R., Rensing, C., Liu, S., Gao, S., Liao, H., Liu, Y. R., Chen, W., Zhu, Y. G., Huang, Q., & Hao, X. (2023). Phagotrophic Protists Modulate Copper Resistance of the Bacterial Community in Soil. Environmental Science and Technology, 57(9), 3590-3601. https://doi.org/10.1021/acs.est.2c07136

Vancouver

Lv Z, Xu M, Liu Y, Rønn R, Rensing C, Liu S et al. Phagotrophic Protists Modulate Copper Resistance of the Bacterial Community in Soil. Environmental Science and Technology. 2023;57(9):3590-3601. https://doi.org/10.1021/acs.est.2c07136

Author

Lv, Zhenguang ; Xu, Min ; Liu, Ying ; Rønn, Regin ; Rensing, Christopher ; Liu, Song ; Gao, Shenghan ; Liao, Hao ; Liu, Yu Rong ; Chen, Wenli ; Zhu, Yong Guan ; Huang, Qiaoyun ; Hao, Xiuli. / Phagotrophic Protists Modulate Copper Resistance of the Bacterial Community in Soil. In: Environmental Science and Technology. 2023 ; Vol. 57, No. 9. pp. 3590-3601.

Bibtex

@article{7107d8491980434a82945a07208a0f8f,

title = "Phagotrophic Protists Modulate Copper Resistance of the Bacterial Community in Soil",

abstract = "Protist predation is a crucial biotic driver modulating bacterial populations and functional traits. Previous studies using pure cultures have demonstrated that bacteria with copper (Cu) resistance exhibited fitness advantages over Cu-sensitive bacteria under the pressure of protist predation. However, the impact of diverse natural communities of protist grazers on bacterial Cu resistance in natural environments remains unknown. Here, we characterized the communities of phagotrophic protists in long-term Cu-contaminated soils and deciphered their potential ecological impacts on bacterial Cu resistance. Long-term field Cu pollution increased the relative abundances of most of the phagotrophic lineages in Cercozoa and Amoebozoa but reduced the relative abundance of Ciliophora. After accounting for soil properties and Cu pollution, phagotrophs were consistently identified as the most important predictor of the Cu-resistant (CuR) bacterial community. Phagotrophs positively contributed to the abundance of a Cu resistance gene (copA) through influencing the cumulative relative abundance of Cu-resistant and -sensitive ecological clusters. Microcosm experiments further confirmed the promotion effect of protist predation on bacterial Cu resistance. Our results indicate that the selection by protist predation can have a strong impact on the CuR bacterial community, which broadens our understanding of the ecological function of soil phagotrophic protists.",

keywords = "bacterial copper (Cu) resistance, copA gene, Cu contamination, soil phagotrophic protists, trophic regulation",

author = "Zhenguang Lv and Min Xu and Ying Liu and Regin R{\o}nn and Christopher Rensing and Song Liu and Shenghan Gao and Hao Liao and Liu, {Yu Rong} and Wenli Chen and Zhu, {Yong Guan} and Qiaoyun Huang and Xiuli Hao",

note = "Publisher Copyright: {\textcopyright} 2023 American Chemical Society.",

year = "2023",

doi = "10.1021/acs.est.2c07136",

language = "English",

volume = "57",

pages = "3590--3601",

journal = "Environmental Science & Technology",

issn = "0013-936X",

publisher = "American Chemical Society",

number = "9",

}

RIS

TY - JOUR

T1 - Phagotrophic Protists Modulate Copper Resistance of the Bacterial Community in Soil

AU - Lv, Zhenguang

AU - Xu, Min

AU - Liu, Ying

AU - Rønn, Regin

AU - Rensing, Christopher

AU - Liu, Song

AU - Gao, Shenghan

AU - Liao, Hao

AU - Liu, Yu Rong

AU - Chen, Wenli

AU - Zhu, Yong Guan

AU - Huang, Qiaoyun

AU - Hao, Xiuli

PY - 2023

Y1 - 2023

N2 - Protist predation is a crucial biotic driver modulating bacterial populations and functional traits. Previous studies using pure cultures have demonstrated that bacteria with copper (Cu) resistance exhibited fitness advantages over Cu-sensitive bacteria under the pressure of protist predation. However, the impact of diverse natural communities of protist grazers on bacterial Cu resistance in natural environments remains unknown. Here, we characterized the communities of phagotrophic protists in long-term Cu-contaminated soils and deciphered their potential ecological impacts on bacterial Cu resistance. Long-term field Cu pollution increased the relative abundances of most of the phagotrophic lineages in Cercozoa and Amoebozoa but reduced the relative abundance of Ciliophora. After accounting for soil properties and Cu pollution, phagotrophs were consistently identified as the most important predictor of the Cu-resistant (CuR) bacterial community. Phagotrophs positively contributed to the abundance of a Cu resistance gene (copA) through influencing the cumulative relative abundance of Cu-resistant and -sensitive ecological clusters. Microcosm experiments further confirmed the promotion effect of protist predation on bacterial Cu resistance. Our results indicate that the selection by protist predation can have a strong impact on the CuR bacterial community, which broadens our understanding of the ecological function of soil phagotrophic protists.

AB - Protist predation is a crucial biotic driver modulating bacterial populations and functional traits. Previous studies using pure cultures have demonstrated that bacteria with copper (Cu) resistance exhibited fitness advantages over Cu-sensitive bacteria under the pressure of protist predation. However, the impact of diverse natural communities of protist grazers on bacterial Cu resistance in natural environments remains unknown. Here, we characterized the communities of phagotrophic protists in long-term Cu-contaminated soils and deciphered their potential ecological impacts on bacterial Cu resistance. Long-term field Cu pollution increased the relative abundances of most of the phagotrophic lineages in Cercozoa and Amoebozoa but reduced the relative abundance of Ciliophora. After accounting for soil properties and Cu pollution, phagotrophs were consistently identified as the most important predictor of the Cu-resistant (CuR) bacterial community. Phagotrophs positively contributed to the abundance of a Cu resistance gene (copA) through influencing the cumulative relative abundance of Cu-resistant and -sensitive ecological clusters. Microcosm experiments further confirmed the promotion effect of protist predation on bacterial Cu resistance. Our results indicate that the selection by protist predation can have a strong impact on the CuR bacterial community, which broadens our understanding of the ecological function of soil phagotrophic protists.

KW - bacterial copper (Cu) resistance

KW - copA gene

KW - Cu contamination

KW - soil phagotrophic protists

KW - trophic regulation

U2 - 10.1021/acs.est.2c07136

DO - 10.1021/acs.est.2c07136

M3 - Journal article

C2 - 36811608

AN - SCOPUS:85149866801

VL - 57

SP - 3590

EP - 3601

JO - Environmental Science & Technology

JF - Environmental Science & Technology

SN - 0013-936X

IS - 9

ER -

ID: 339729441

Department of Biology