Metabolic Regulation Lab

We study adipocytes (fat cells) and adipose tissue. In recent years, our focus has been on the molecular control of differentiation, activation and metabolism of brown adipocytes. Our interest in brown adipose tissue biology derives from the striking ability of brown adipocytes to counteract obesity and insulin resistance in experimental animals and the observation that active brown fat is present in many adult humans. Ongoing projects revolve around metabolic regulation and signal transduction. Our aim is to uncover novel regulatory mechanisms through which brown adipose tissue can be exploited therapeutically.



























J. R. Alvarez-Dominguez, S. Winther, J. B. Hansen, H. F. Lodish, M. Knoll.
An adipose lncRAP2-Igf2bp2 complex enhances adipogenesis and energy expenditure by stabilizing target mRNAs.
iScience 25(1), 103680. Link


O. Sveidahl Johansen, T. Ma, J. B. Hansen, L. K. Markussen, R. Schreiber, L. Reverte-Salisa, H. Dong, D. P. Christensen, W. Sun, T. Gnad, I. Karavaeva, T. S. Nielsen, S. Kooijman, C. Cero, O. Dmytriyeva, Y. Shen, M. Razzoli, S. L. O'Brien, E. N. Kuipers, C. H. Nielsen, W. Orchard, N. Willemsen, N. Z. Jespersen, M. Lundh, E. G. Sustarsic, C. M. Hallgren, M. Frost, S. McGonigle, M. S. Isidor, C. Broholm, O. Pedersen, J. B. Hansen, N. Grarup, T. Hansen, A. Kjær, J. G. Granneman, M. M. Babu, D. Calebiro, S. Nielsen, M. Ryden, R. Soccio, P. C. N. Rensen, J. T. Treebak, T. W. Schwartz, B. Emanuelli, A. Bartolomucci, A. Pfeifer, R. Zechner, C. Scheele, S. Mandrup, Z. Gerhart-Hines.
Lipolysis drives expression of the constitutively active GPR3 to induce adipose thermogenesis.
Cell 184(13), 3502-3518. 2021. Link

J. X. Shen, M. Couchet, J. Dufau, T. de Castro Barbosa, M. H. Ulbrich, M. Helmstadter, A. M. Kemas, R. Zandi Shafagh, M. A. Marques, J. B. Hansen, N. Mejhert, D. Langin, M. Ryden, V. M. Lauschke.
3D adipose tissue culture links the organotypic microenvironment to improved adipogenesis.
Adv. Sci.: e2100106. 2021. Link

P. Breining, S. B. Pedersen, M. Kjølby, J. B. Hansen, N. Jessen, B. Richelsen.
Parathyroid hormone receptor stimulation induces human adipocyte lipolysis and browning.
Eur. J. Endocrinol. 184(5), 687-697. 2021. Link


S. Justesen. K. V. Haugegaard, J. B. Hansen, H. S. Hansen, B. Andersen.
The autocrine role of FGF21 in cultured adipocytes.
Biochem. J. 477(13), 2477-87. 2020. Link

M. S. Isidor, S. Winther, L. K. Markussen, A. L. Basse, B. Quistorff, J. Nedergaard, B. Emanuelli, J. B. Hansen.*
Pyruvate kinase M2 represses thermogenic gene expression in brown adipocytes.
FEBS Lett. 594(7):1218-1225. 2020. Link

M. J. Riis-Vestergaard, B. Richelsen, J. M. Bruun, W. Li, J. B. Hansen, S. B. Pedersen.
Beta-1 and not beta-3 adrenergic receptors may be the primary regulator of human brown adipocyte metabolism.
J. Clin. Endocrinol. Metab. 105(4). 2020. Link

M. Tozzi, J. B. Hansen, I. Novak.
Pannexin-1 mediated ATP release in adipocytes is sensitive to glucose and insulin and modulates lipolysis and macrophage migration.
Acta Physiol. 228(2):e13360. 2020. Link


N. B. Danneskiold-Samsøe, S. B. Sonne, J. M. Larsen, A. N. Hansen, E. Fjære, M. S. Isidor, S. Petersen, J. Henningsen, I. Severi, L. Sartini, Y. Schober, J. Wolf, W. A. Nockher, C. Wolfrum, S. Cinti, C. Sina, J. B. Hansen, L. Madsen, S. Brix, K. Kristiansen.
Overexpression of cyclooxygenase-2 in adipocytes reduces fat accumulation in inguinal white adipose tissue and hepatic steatosis in high-fat fed mice.
Sci. Rep. 9, 8979. 2019. Link

L. J. Mortensen, M. Lorenzen, N. Jørgensen, A. M. Andersson, J. E. Nielsen, L. I. Petersen, B. Lanske, A. Juul, J. B. Hansen, M. B. Jensen.
Possible link between FSH and RANKL release from adipocytes in men with impaired gonadal function including Klinefelter syndrome.
Bone 123, 103-114. 2019. Link


L. K. Markussen, S. Winther, B. Wicksteed, J. B. Hansen.*
GSK3 is a negative regulator of the thermogenic program in brown adipocytes.
Sci. Rep. 8, 3469. 2018. Link

S. Winther, M. S. Isidor, A. L. Basse, N. Skjoldborg, A. Cheung, B. Quistorff, J. B. Hansen.*
Restricting glycolysis impairs brown adipocyte glucose and oxygen consumption.
Am. J. Physiol. Endocrinol. Metab. 314, E214-E223. 2018. Link

P. Breining, J. B. Jensen, E. I Gormsen, S. Jakobsen, M. Busk, L. Rolighed, P. Bross, P. Fernandez-Guerra, L. K. Markussen, N. E. Rasmussen, J. B. Hansen, S. B. Pedersen, B. Richelsen, N. Jessen.
Metformin targets brown adipose tissue in vivo and reduces oxygen consumption in vitro.
Diabetes Obes. Metab. 20, 2264-2273. 2018. Link


M. Knoll, S. Winther, A. Natarajan, H. Yang, M. Jiang, P. Thiru, A. Shahsafaei, T. E. Chavarria, D. W. Lamming, L. Sun, J. B. Hansen, H. F. Lodish.
SYK kinase mediates brown fat differentiation and activation.
Nat. Commun. 8, 2115. 2017. Link

C. Petersen, M. D. Nielsen, E. S. Andersen, A. L. Basse, M. S. Isidor, L. K. Markussen, B. M. Viuff, I. H. Lambert. J. B. Hansen, S. F. Pedersen.
MCT1 and MCT4 expression and lactate flux activity increase during white and brown adipogenesis and impact adipocyte metabolism.
Sci. Rep. 7, 13101. 2017. Link

A. L. Basse, M. S. Isidor, S. Winther, N. B. Skjoldborg, M. Murholm, E. S. Andersen, S. B. Pedersen, C. Wolfrum, B. Quistorff, J. B. Hansen.*
Regulation of glycolysis in brown adipocytes by HIF-1α.
Sci. Rep. 7, 4052. 2017. Link

L. K. Markussen, M. S. Isidor, P. Breining, E. S. Andersen, N. E. Rasmussen, L. I. Petersen, S. B. Pedersen, B. Richelsen, J. B. Hansen.*
Characterization of immortalized human brown and white pre-adipocyte cell models from a single donor.
PLoS ONE 12(9):e0185624. 2017. Link


M. S. Isidor, S. Winther, A. L. Basse, M. C. H. Petersen, B. Cannon, J. Nedergaard, J. B. Hansen.*
An siRNA-based method for efficient silencing of gene expression in mature brown adipocytes.
Adipocyte 5, 175-185. 2016. Link

P. Hallenborg, E. Fjære, B. Liaset, R. K. Petersen, I. Murano, S. B. Sonne, M. Falkerslev, S. Winther, B. A. H. Jensen, T. Ma, J. B. Hansen, S. Cinti, B. Blagoev, L. Madsen, K. Kristiansen
p53 regulates expression of uncoupling protein 1 through binding and repression of PPARγ coactivator 1α.
Am. J. Physiol. Endocrinol. Metab. 310, E116-128. 2016. Link

U. Liisberg, L. Myrmel, E. Fjære, A. Rønnevik, S. Bjelland, K. Fauske, J. B. Holm, A. L. Basse, J. B. Hansen, B. Liaset, K. Kristiansen, L. Madsen.
The protein source determines the potential of high protein diets to attenuate obesity development in C57BL/6J mice.
Adipocyte 5, 196-211. 2016. Link


A. L. Basse, K. Dixen, R. Yadav, M. P. Tygesen, K. Qvortrup, K. Kristiansen, B. Quistorff, R. Gupta,* J. Wang, J. B. Hansen.*
Global gene expression profiling of brown to white adipose tissue transformation in sheep reveals novel transcriptional components linked to adipose remodeling.
BMC Genomics 16, 215. 2015. Link

Q. Hao, R. Yadav, A. L. Basse, S. Petersen, S. B. Sonne, S. Rasmussen, Q. Zhu, Z. Lu, J. Wang, K. Audouze, R. Gupta, L. Madsen, K. Kristiansen, J. B. Hansen.*
Transcriptome profiling of brown adipose tissue during cold exposure reveals extensive regulation of glucose metabolism.
Am. J. Physiol. Endocrinol. Metab. 308, E380-E392. 2015. Link

J. Pingel, M. C. H. Petersen, U. Fredberg, S. G. Kjær, B. Quistorff, H. Langberg, J. B. Hansen.*
Inflammatory and metabolic alterations of Kager’s fat pad in chronic Achilles tendinopathy.
PLoS ONE 10:e0127811. 2015. Link

T. L. Vormer, J. B. Hansen, H. te Riele.
The retinoblastoma protein: multi-tasking to suppress tumorigenesis.
Mol. Cell. Oncol. 2:1, e968062. 2015. Link


J. G. Knudsen, M. Murholm, A. L. Carey, R. S. Biensø, A. L. Basse, T. L. Allen, J. Hidalgo, B. A. Kingwell, M. A. Febbraio, J. B. Hansen, H. Pilegaard.
Role of IL-6 in exercise training- and cold-induced UCP1 expression in subcutaneous white adipose tissue.
PLoS ONE 9: e84910. 2014. Link

Y. Jiang, M. Xie, W. Chen, R. Talbot, J. F. Maddox, T. Faraut, C. Wu, D. M. Muzny, Y. Li, W. Zhang, J. A. Stanton, R. Brauning, W. C. Barris, T. Hourlier, B. L. Aken, S. M. Searle, D. L. Adelson, C. Bian, G. R. Cam, Y. Chen, S. Cheng, U. DeSilva, K. Dixen, Y. Dong, G. Fan, I. R. Franklin, S. Fu, P. Fuentes-Utrilla, R. Guan, M. A. Highland, M. E. Holder, G. Huang, A. B. Ingham, S. N. Jhangiani, D. Kalra, C. L. Kovar, S. L. Lee, W. Liu, X. Liu, C. Lu, T. Lv, T. Mathew, S. McWilliam, M. Menzies, S. Pan, D. Robelin, B. Servin, D. Townley, W. Wang, B. Wei, S. N. White, X. Yang, C. Ye, Y. Yue, P. Zeng, Q. Zhou, J. B. Hansen, K. Kristiansen, R. A. Gibbs, P. Flicek, C. C. Warkup, H. E. Jones, V. H. Oddy, F. W. Nicholas, J. C. McEwan, J. W. Kijas, J. Wang, K. C. Worley, A. L. Archibald, N. Cockett, X. Xu, W. Wang, B. P. Dalrymple.
The sheep genome illuminates biology of the rumen and lipid metabolism.
Science 344, 1168-1173. 2014. Link

T. L. Vormer, K. Wojciechowicz, M. Dekker, S. de Vries, A. van der Wal, E. Delzenne-Goette, S. H. Naik, J.-Y. Song, J.-H. Dannenberg, J. B. Hansen,* H. te Riele.*
RB family tumor suppressor activity may not relate to active silencing of E2F target genes.
Cancer Res. 74, 5266-5276. 2014. Link

P. Hallenborg, R. K. Petersen, S. Feddersen, U. Sundekilde, J. B. Hansen, B. Blagoev, L. Madsen, K. Kristiansen.
PPARγ ligand production is tightly linked to clonal expansion during initiation of adipocyte differentiation.
J. Lipid Res. 55, 2491-2500. 2014. Link


K. Dixen, A. L. Basse, M. Murholm, M. S. Isidor, L. H. L. Hansen, M. C. H. Petersen, L. Madsen, N. Petrovic, J. Nedergaard, B. Quistorff, J. B. Hansen.*
ERRγ enhances UCP1 expression and fatty acid oxidation in brown adipocytes.
Obesity 21, 516-524. 2013. Link

M. Murholm, M. S. Isidor, A. L. Basse, S. Winther, C. Sørensen, J. Skovgaard-Petersen, M. M. Nielsen, A. S. Hansen, B. Quistorff, J. B. Hansen.*
Retinoic acid has different effects on UCP1 expression in mouse and human adipocytes.
BMC Cell Biol. 14, 41. 2013. Link

N.-E. Viby, M. S. Isidor, K. B. Buggeskov, S. S. Poulsen, J. B. Hansen, H. Kissow.
Glucagon-like peptide-1 (GLP-1) reduces mortality and improves lung function in a model of experimental obstructive lung disease in female mice.
Endocrinology 154, 4503-4511. 2013. Link

Selected publications before 2013:

R. Hakim-Weber, A. M. Krogsdam, C. Jørgensen, M. Fischer, A. Prokesch, J. G.. Bogner-Strauss, S. R. Bornstein, J. B. Hansen, L. Madsen, K. Kristiansen, Trajanoski Z, Hackl H.
Transcriptional regulatory program in wild-type and retinoblastoma gene-deficient mouse embryonic fibroblasts during adipocyte differentiation.
BMC Res. Notes 26;4:157. 2011. Link

D. M. Kristensen, M. L. Skalkam, K. Audouze, L. Lesné, C. Desdoits-Lethimonier, H. Frederiksen, S. Brunak, N. E. Skakkebæk, B. Jégou, J. B. Hansen, S. Junker, H. Leffers.
Many putative endocrine disruptors inhibit prostaglandin synthesis.
Environ. Health Perspect. 119:534-541. 2011. Link

L. Madsen, L. M. Pedersen, H. H. Lillefosse, E. Fjære, I. Bronstad, Q. Hao, R. K. Petersen, P. Hallenborg, T. Ma, R. D. Matteis, P. Araujo, J. Mercader, M. L. Bonet, J. B. Hansen, B. Cannon, J. Nedergaard, J. Wang, S. Cinti, P. Voshol, S. O. Døskeland, K. Kristiansen.
UCP1 induction during recruitment of brown adipocytes in white adipose tissue is dependent on cyclooxygenase activity.
PLoS ONE 5, e11391. 2010. Link

M. Murholm, K. Dixen, J. B. Hansen.*
Ras signalling regulates differentiation and UCP1 expression in models of brown adipogenesis.
Biochim. Biophys. Acta 1800, 619-627. 2010. Link

Q. Hao, J. B. Hansen, R. K. Petersen, P. Hallenborg, C. Jørgensen, S. Cinti, P. J. Larsen, K. R. Steffensen, H. Wang, S. Collins, J. Wang, J.-Å. Gustafsson, L. Madsen, K. Kristiansen.
ADD1/SREBP1c activates the PGC1α promoter in brown adipocytes.
Biochim. Biophys. Acta 1801, 421-429. 2010. Link

M. Murholm, K. Dixen, K. Qvortrup, L. H. L. Hansen, E.-Z. Amri, L. Madsen, G. Barbatelli, B. Quistorff, J. B. Hansen.*
Dynamic regulation of genes involved in mitochondrial DNA replication and transcription during mouse brown fat cell differentiation and recruitment.
PLoS ONE 4, e8458. 2009. Link

D. Pasini, A. P. Bracken, J. B. Hansen, M. Capillo, K. Helin.
The polycomb group protein Suz12 is required for embryonic stem cell differentiation.
Mol. Cell. Biol. 27, 3769-3779. 2007. Link

K. L. Egerod, B. Holst, P. S. Petersen, J. B. Hansen, J. Mulder, T. Hökfelt, T. W. Schwarz.
GPR39 splice variants versus antisense gene LYPD1: Expression and regulation in gastrointestinal tract, endocrine pancreas, liver and white adipose tissue.
Mol. Endocrinol. 21, 1685-1698. 2007. Link

J. B. Hansen,* K. Kristiansen.*
Pocket proteins control white versus brown fat cell differentiation.
Cell Cycle 5, 341-342. 2006. Link

I. Iankova, R. K. Petersen, J.-S. Annicotte, C. Chavey, J. B. Hansen, I. Kratchmarova, D. Sarruf, M. Benkirane, K. Kristiansen, L. Fajas.
Peroxisome proliferator-activated receptor γ recruits the positive transcription elongation factor b complex to activate transcription and promote adipogenesis.
Mol. Endocrinol. 20, 1494-1505. 2006. Link

J. B. Hansen,* K. Kristiansen.
Regulatory circuits controlling white versus brown adipocyte differentiation.
Biochem. J. 398, 153-168. 2006. Link

S. Kang, L. Bajnok, K. A. Longo, R. K. Petersen, J. B. Hansen, K. Kristiansen, O. A. MacDougald.
Effects of Wnt signaling on brown adipocyte differentiation and metabolism mediated by PGC-1α.
Mol. Cell. Biol. 25, 1272-1282. 2005. Link

J. B. Hansen, C. Jørgensen, R. K. Petersen, P. Hallenborg, R. De Matteis, H. A. Bøye, N. Petrovic, S. Enerbäck, J. Nedergaard, S. Cinti, H. te Riele, K. Kristiansen.
Retinoblastoma protein functions as a molecular switch determining white versus brown adipocyte differentiation.
Proc. Natl. Acad. Sci. USA 101, 4112-4117. 2004. Link

J. B. Hansen, H. te Riele, K. Kristiansen.
Novel function of the retinoblastoma protein in fat: regulation of white versus brown adipocyte differentiation.
Cell Cycle 3, 774-778. 2004. Link

J. B. Hansen, R. K. Petersen, C. Jørgensen, K. Kristiansen.
Deregulated MAPK activity prevents adipocyte differentiation of fibroblasts lacking the retinoblastoma protein.
J. Biol. Chem. 277, 26335-26339. 2002. Link

L. Fajas, V. Egler, R. Reiter, J. Hansen, K. Kristiansen, M.-B. Debril, S. Miard, J. Auwerx.
The retinoblastoma-histone deacetylase 3 complex inhibits PPARγ and adipocyte differentiation.
Dev. Cell 3, 903-910. 2002. Link

J. B. Hansen, H. Zhang, T. H. Rasmussen, R. K. Petersen, K. Kristiansen.
Peroxisome proliferator-activated receptor δ (PPARδ)-mediated regulation of preadipocyte proliferation and gene expression is dependent on cAMP signaling.
J. Biol. Chem. 276, 3175-3182. 2001. Link

J. B. Hansen, R. K. Petersen, B. M. Larsen, J. Bartkova, J. Alsner, K. Kristiansen.
Activation of peroxisome proliferator-activated receptor γ bypasses the function of the retinoblastoma protein in adipocyte differentiation.
J. Biol. Chem. 274, 2386-2393. 1999. Link






























Metabolic Regulation Lab

Cell Biology and Physiology
Universitetsparken 13
DK-2100 Copenhagen Ø, Denmark

Associate Professor Jacob B. Hansen
Phone: +45 4075 9544


June 2021
New co-author papers out!

The first one is a Cell paper from the Gerhart-Hines lab about GPR3 and adipose thermogenesis.

The second one is an Advanced Science paper from the Lauchske lab about the potency of 3D culturing methods to improve human adipogenesis.

May 2021
New paper in collaboration with colleagues from Aarhus University Hospital about the ability of parathyroid hormone and its receptor to regulate human adipocyte lipolysis and thermogenic gene expression is out now!