A cell shrinkage-induced non-selective cation conductance with a novel pharmacology in Ehrlich-Lettre-ascites tumour cells.

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

A cell shrinkage-induced non-selective cation conductance with a novel pharmacology in Ehrlich-Lettre-ascites tumour cells. / Lawonn, Peter; Hoffmann, Else K; Hougaard, Charlotte; Wehner, Frank.

In: FEBS Letters, Vol. 539, No. 1-3, 2003, p. 115-9.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Lawonn, P, Hoffmann, EK, Hougaard, C & Wehner, F 2003, 'A cell shrinkage-induced non-selective cation conductance with a novel pharmacology in Ehrlich-Lettre-ascites tumour cells.', FEBS Letters, vol. 539, no. 1-3, pp. 115-9. https://doi.org/10.1016/S0014-5793(03)00210-2

APA

Lawonn, P., Hoffmann, E. K., Hougaard, C., & Wehner, F. (2003). A cell shrinkage-induced non-selective cation conductance with a novel pharmacology in Ehrlich-Lettre-ascites tumour cells. FEBS Letters, 539(1-3), 115-9. https://doi.org/10.1016/S0014-5793(03)00210-2

Vancouver

Lawonn P, Hoffmann EK, Hougaard C, Wehner F. A cell shrinkage-induced non-selective cation conductance with a novel pharmacology in Ehrlich-Lettre-ascites tumour cells. FEBS Letters. 2003;539(1-3):115-9. https://doi.org/10.1016/S0014-5793(03)00210-2

Author

Lawonn, Peter ; Hoffmann, Else K ; Hougaard, Charlotte ; Wehner, Frank. / A cell shrinkage-induced non-selective cation conductance with a novel pharmacology in Ehrlich-Lettre-ascites tumour cells. In: FEBS Letters. 2003 ; Vol. 539, No. 1-3. pp. 115-9.

Bibtex

@article{71fac5a0a0f311dd86a6000ea68e967b,
title = "A cell shrinkage-induced non-selective cation conductance with a novel pharmacology in Ehrlich-Lettre-ascites tumour cells.",
abstract = "In whole-cell recordings on Ehrlich-Lettre-ascites tumour (ELA) cells, the shrinkage-induced activation of a cation conductance with a selectivity ratio P(Na):P(Li):P(K):P(choline):P(NMDG) of 1.00:0.97:0.88:0.03:0.01 was observed. In order of potency, this conductance was blocked by Gd(3+)=benzamil>amiloride>ethyl-isopropyl-amiloride (EIPA). In patch-clamp studies using the cell-attached configuration, a 14 pS channel became detectable that was reversibly activated upon hypertonic cell shrinkage. It is concluded that ELA cells express a shrinkage-induced cation channel that may reflect a molecular link between amiloride-sensitive and -insensitive channels. In addition, because of its pharmacological profile, it may possibly be related to epithelial Na+ channels (ENaCs).",
author = "Peter Lawonn and Hoffmann, {Else K} and Charlotte Hougaard and Frank Wehner",
note = "Keywords: Animals; Carcinoma, Ehrlich Tumor; Cations; Cell Size; Electric Conductivity; Ion Channels; Patch-Clamp Techniques; Tumor Cells, Cultured",
year = "2003",
doi = "10.1016/S0014-5793(03)00210-2",
language = "English",
volume = "539",
pages = "115--9",
journal = "F E B S Letters",
issn = "0014-5793",
publisher = "JohnWiley & Sons Ltd",
number = "1-3",

}

RIS

TY - JOUR

T1 - A cell shrinkage-induced non-selective cation conductance with a novel pharmacology in Ehrlich-Lettre-ascites tumour cells.

AU - Lawonn, Peter

AU - Hoffmann, Else K

AU - Hougaard, Charlotte

AU - Wehner, Frank

N1 - Keywords: Animals; Carcinoma, Ehrlich Tumor; Cations; Cell Size; Electric Conductivity; Ion Channels; Patch-Clamp Techniques; Tumor Cells, Cultured

PY - 2003

Y1 - 2003

N2 - In whole-cell recordings on Ehrlich-Lettre-ascites tumour (ELA) cells, the shrinkage-induced activation of a cation conductance with a selectivity ratio P(Na):P(Li):P(K):P(choline):P(NMDG) of 1.00:0.97:0.88:0.03:0.01 was observed. In order of potency, this conductance was blocked by Gd(3+)=benzamil>amiloride>ethyl-isopropyl-amiloride (EIPA). In patch-clamp studies using the cell-attached configuration, a 14 pS channel became detectable that was reversibly activated upon hypertonic cell shrinkage. It is concluded that ELA cells express a shrinkage-induced cation channel that may reflect a molecular link between amiloride-sensitive and -insensitive channels. In addition, because of its pharmacological profile, it may possibly be related to epithelial Na+ channels (ENaCs).

AB - In whole-cell recordings on Ehrlich-Lettre-ascites tumour (ELA) cells, the shrinkage-induced activation of a cation conductance with a selectivity ratio P(Na):P(Li):P(K):P(choline):P(NMDG) of 1.00:0.97:0.88:0.03:0.01 was observed. In order of potency, this conductance was blocked by Gd(3+)=benzamil>amiloride>ethyl-isopropyl-amiloride (EIPA). In patch-clamp studies using the cell-attached configuration, a 14 pS channel became detectable that was reversibly activated upon hypertonic cell shrinkage. It is concluded that ELA cells express a shrinkage-induced cation channel that may reflect a molecular link between amiloride-sensitive and -insensitive channels. In addition, because of its pharmacological profile, it may possibly be related to epithelial Na+ channels (ENaCs).

U2 - 10.1016/S0014-5793(03)00210-2

DO - 10.1016/S0014-5793(03)00210-2

M3 - Journal article

C2 - 12650937

VL - 539

SP - 115

EP - 119

JO - F E B S Letters

JF - F E B S Letters

SN - 0014-5793

IS - 1-3

ER -

ID: 6768745