Co-expression of mCysLT1 receptors and IK channels in Xenopus laevis oocytes elicits LTD4-stimulated IK current, independent of an increase in [Ca2+]i.
Research output: Contribution to journal › Journal article › Research › peer-review
Addition of LTD4 (10 nM) to Xenopus laevis oocytes expressing the mCysLT1 receptor together with hBK or hIK channels resulted in the activation of both channels secondary to an LTD4-induced increase in [Ca2+]i. In addition, the hIK channel is activated by low concentrations of LTD4 (<0.1 nM), which did not result in any increase in [Ca2+]i. Even though activation of hIK by low concentrations of LTD4 was independent of an increase in [Ca2+]i, a certain "permissive" level of [Ca2+]i was required for its activation, since buffering of intracellular Ca2+ by EGTA completely abolished the response to LTD4. Neither hTBAK1 nor hTASK2 was activated following stimulations with LTD4 (0.1 and 100 nM).
Original language | English |
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Journal | BBA General Subjects |
Volume | 1660 |
Issue number | 1-2 |
Pages (from-to) | 75-9 |
Number of pages | 4 |
ISSN | 0304-4165 |
DOIs | |
Publication status | Published - 2004 |
Bibliographical note
Keywords: Animals; Calcium; Cations, Divalent; Cell Line; Cytokines; Egtazic Acid; Humans; Hydrogen-Ion Concentration; Large-Conductance Calcium-Activated Potassium Channels; Leukotriene D4; Membrane Proteins; Oocytes; Potassium Channels; Potassium Channels, Calcium-Activated; Potassium Channels, Tandem Pore Domain; RNA, Complementary; Receptors, Leukotriene; Transfection; Xenopus laevis
ID: 6768716