Isolation and differentiation of stromal vascular cells to beige/brite cells
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Isolation and differentiation of stromal vascular cells to beige/brite cells. / Aune, Ulrike Liisberg; Ruiz, Lauren; Kajimura, Shingo.
In: Journal of Visualized Experiments, No. 73, e50191, 2013.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Isolation and differentiation of stromal vascular cells to beige/brite cells
AU - Aune, Ulrike Liisberg
AU - Ruiz, Lauren
AU - Kajimura, Shingo
PY - 2013
Y1 - 2013
N2 - Brown adipocytes have the ability to uncouple the respiratory chain in mitochondria and dissipate chemical energy as heat. Development of UCP1-positive brown adipocytes in white adipose tissues (so called beige or brite cells) is highly induced by a variety of environmental cues such as chronic cold exposure or by PPARγ agonists, therefore, this cell type has potential as a therapeutic target for obesity treatment. Although most immortalized adipocyte lines cannot recapitulate the process of "browning" of white fat in culture, primary adipocytes isolated from stromal vascular fraction in subcutaneous white adipose tissue (WAT) provide a reliable cellular system to study the molecular control of beige/brite cell development. Here we describe a protocol for effective isolation of primary preadipocytes and for inducing differentiation to beige/brite cells in culture. The browning effect can be assessed by the expression of brown fat-selective markers such as UCP1.
AB - Brown adipocytes have the ability to uncouple the respiratory chain in mitochondria and dissipate chemical energy as heat. Development of UCP1-positive brown adipocytes in white adipose tissues (so called beige or brite cells) is highly induced by a variety of environmental cues such as chronic cold exposure or by PPARγ agonists, therefore, this cell type has potential as a therapeutic target for obesity treatment. Although most immortalized adipocyte lines cannot recapitulate the process of "browning" of white fat in culture, primary adipocytes isolated from stromal vascular fraction in subcutaneous white adipose tissue (WAT) provide a reliable cellular system to study the molecular control of beige/brite cell development. Here we describe a protocol for effective isolation of primary preadipocytes and for inducing differentiation to beige/brite cells in culture. The browning effect can be assessed by the expression of brown fat-selective markers such as UCP1.
KW - Adipocytes
KW - Adipose tissue
KW - Anatomy
KW - Animal model
KW - Beige/brite cells
KW - Brown adipose tissue
KW - Cell Differentiation
KW - Cells
KW - Cellular biology
KW - Diabetes
KW - Differentiation
KW - Fat
KW - Isolation
KW - Issue 73
KW - Medicine
KW - Molecular biology
KW - Obesity
KW - Physiology
KW - Primary adipocytes
KW - Rosiglitazone
KW - Stromal-vascular fraction
KW - Surgery
KW - Transcription factors
KW - Uncoupling protein 1
U2 - 10.3791/50191
DO - 10.3791/50191
M3 - Journal article
C2 - 23568137
AN - SCOPUS:84875895887
JO - Journal of Visualized Experiments
JF - Journal of Visualized Experiments
SN - 1940-087X
IS - 73
M1 - e50191
ER -
ID: 120025049