Isolation of <Glu-Asn-Phe-His-Leu-Arg-Pro-NH2 (Antho-RPamide II), a novel, biologically active neuropeptide from sea anemones
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Using a radioimmunoassay against the C-terminal sequence Arg-Pro-NH2 (RPamide) we have isolated the neuropeptide <Glu-Asn-Phe-His-Leu-Arg-Pro-NH2 (Antho-RPamide II) from extracts of the sea anemone Anthopleura elegantissima. Addition of low concentrations of Antho-RPamide II to a tentacle preparation of sea anemones inhibited the spontaneous, rhythmic contractions, suggesting that the peptide is a neurotransmitter or neuromodulator.
| Original language | English |
|---|---|
| Journal | Peptides |
| Volume | 14 |
| Issue number | 2 |
| Pages (from-to) | 131-135 |
| Number of pages | 5 |
| ISSN | 0196-9781 |
| DOIs | |
| Publication status | Published - 1993 |
Bibliographical note
Funding Information:
We thank Susanne Raabe and Dagmar Boshold for typing the manuscript, and the Bundesministerium f'dr Forschung und Technologie, the Deutsche Forschungsgemeinschafi (Gr 762/7-3), Fonds der Chemischen lndustrie (to C.J.P.G.), NATO (Collaborative Research Grant to C.J.P.G.), Ciba-Geigy (Award for Cooperation in Europe to I.D.M.), and the National Institute of General Medical Sciences (GM 27029, to K.L.R.) for financial support. A part of this article is based on a doctoral study by K.C. in the Faculty of Biology, University of Hamburg.
- Coelenterate, Mass spectrometry, Neurohormone, Neurotransmitter, Peptide structure
Research areas
ID: 370739696