Genomic structure of bacteriophage 6H and its distribution as prophage in Flavobacterium psychrophilum strains
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
Genomic structure of bacteriophage 6H and its distribution as prophage in Flavobacterium psychrophilum strains. / Castillo Bermúdez, Daniel Elías; Espejo, Romilio; Middelboe, Mathias.
In: FEMS Microbiology Letters, Vol. 351, No. 1, 2014, p. 51-58.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Genomic structure of bacteriophage 6H and its distribution as prophage in Flavobacterium psychrophilum strains
AU - Castillo Bermúdez, Daniel Elías
AU - Espejo, Romilio
AU - Middelboe, Mathias
PY - 2014
Y1 - 2014
N2 - Flavobacterium psychrophilum is currently one of the most devastating fish pathogens worldwide causing considerable economic losses in salmonid aquaculture. Recently, attention has been drawn to the use of phages for controlling F. psychrophilum, and phages infecting the pathogen have been isolated. Here, we present the genome sequence of F. psychrophilum bacteriophage 6H and its distribution as prophage in F. psychrophilum isolates. The DNA sequence revealed a genome of 46 978 bp containing 63 predicted ORFs, of which 13% was assigned a putative function, including an integrase. Sequence analysis showed > 80% amino acid similarity to a specific region found in the virulent F. psychrophilum strain JIP02/86 (ATCC 49511), suggesting that a prophage similar to phage 6H was present in this strain. Screening for a collection of 49 F. psychrophilum strains isolated in Chile, Denmark, and USA for the presence of four phage 6H genes (integrase, tail tape protein and two hypothetical proteins) by PCR showed the presence of these prophage genes in 80% of the isolates. In conclusion, we hypothesize that bacteriophage 6H belongs to an abundant group of temperate phages which has lysogenized a large fraction of the global F. psychrophilum community.
AB - Flavobacterium psychrophilum is currently one of the most devastating fish pathogens worldwide causing considerable economic losses in salmonid aquaculture. Recently, attention has been drawn to the use of phages for controlling F. psychrophilum, and phages infecting the pathogen have been isolated. Here, we present the genome sequence of F. psychrophilum bacteriophage 6H and its distribution as prophage in F. psychrophilum isolates. The DNA sequence revealed a genome of 46 978 bp containing 63 predicted ORFs, of which 13% was assigned a putative function, including an integrase. Sequence analysis showed > 80% amino acid similarity to a specific region found in the virulent F. psychrophilum strain JIP02/86 (ATCC 49511), suggesting that a prophage similar to phage 6H was present in this strain. Screening for a collection of 49 F. psychrophilum strains isolated in Chile, Denmark, and USA for the presence of four phage 6H genes (integrase, tail tape protein and two hypothetical proteins) by PCR showed the presence of these prophage genes in 80% of the isolates. In conclusion, we hypothesize that bacteriophage 6H belongs to an abundant group of temperate phages which has lysogenized a large fraction of the global F. psychrophilum community.
UR - http://www.scopus.com/inward/record.url?scp=84893769951&partnerID=8YFLogxK
U2 - 10.1111/1574-6968.12342
DO - 10.1111/1574-6968.12342
M3 - Journal article
C2 - 24267868
AN - SCOPUS:84893769951
VL - 351
SP - 51
EP - 58
JO - F E M S Microbiology Letters
JF - F E M S Microbiology Letters
SN - 0378-1097
IS - 1
ER -
ID: 111065297