In Vitro Evolution of Specific Phages Infecting the Fish Pathogen Flavobacterium psychrophilum
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
In Vitro Evolution of Specific Phages Infecting the Fish Pathogen Flavobacterium psychrophilum. / Castillo, Daniel; Højsting, Alexander Ravndal; Roosvall, Andrea; Smyrlis, Giorgos; Jørgensen, Johanna; Middelboe, Mathias.
In: PHAGE: Therapy, Applications, and Research, Vol. 3, No. 1, 2022, p. 28-37.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - In Vitro Evolution of Specific Phages Infecting the Fish Pathogen Flavobacterium psychrophilum
AU - Castillo, Daniel
AU - Højsting, Alexander Ravndal
AU - Roosvall, Andrea
AU - Smyrlis, Giorgos
AU - Jørgensen, Johanna
AU - Middelboe, Mathias
N1 - Publisher Copyright: © 2022, Mary Ann Liebert, Inc., publishers 2022.
PY - 2022
Y1 - 2022
N2 - Background: Flavobacterium psychrophilum is the causative agent of the bacterial cold-water disease and rainbow trout fry syndrome. Owing to the issues associated with increasing use of antibiotics to control the diseases, phage therapy has been proposed as an alternative method to control Flavobacterium infection within the industry. Materials and Methods: We explored two simple and fast in vitro strategies for the isolation of evolved F. psychrophilum phages, using three well-characterized phages FpV4, FpV9, and FPSV-S20. Results: During in vitro serial transfer experiments, 12 evolved phages were selected 72-96 h after phage exposure in the first or second week. Phenotype analysis showed improvement of host range and efficiency of plating and adsorption constants. Comparative genomic analysis of the evolved phages identified 13 independent point mutations causing amino acid changes mostly in hypothetical proteins. Conclusions: These results confirmed the reliability and effectivity of two strategies to isolate evolved F. psychrophilum phages, which may be used to expand phage-host range and target phage-resistant pathogens in phage therapy applications against Flavobacterium infections.
AB - Background: Flavobacterium psychrophilum is the causative agent of the bacterial cold-water disease and rainbow trout fry syndrome. Owing to the issues associated with increasing use of antibiotics to control the diseases, phage therapy has been proposed as an alternative method to control Flavobacterium infection within the industry. Materials and Methods: We explored two simple and fast in vitro strategies for the isolation of evolved F. psychrophilum phages, using three well-characterized phages FpV4, FpV9, and FPSV-S20. Results: During in vitro serial transfer experiments, 12 evolved phages were selected 72-96 h after phage exposure in the first or second week. Phenotype analysis showed improvement of host range and efficiency of plating and adsorption constants. Comparative genomic analysis of the evolved phages identified 13 independent point mutations causing amino acid changes mostly in hypothetical proteins. Conclusions: These results confirmed the reliability and effectivity of two strategies to isolate evolved F. psychrophilum phages, which may be used to expand phage-host range and target phage-resistant pathogens in phage therapy applications against Flavobacterium infections.
KW - F. psychrophilum
KW - phage evolution
KW - phage mutations
KW - phage-host range
U2 - 10.1089/phage.2022.0006
DO - 10.1089/phage.2022.0006
M3 - Journal article
C2 - 37025924
AN - SCOPUS:85132031714
VL - 3
SP - 28
EP - 37
JO - PHAGE: Therapy, Applications, and Research
JF - PHAGE: Therapy, Applications, and Research
SN - 2641-6530
IS - 1
ER -
ID: 322279227