Production and transformation of dissolved neutral sugars and amino acids by bacteria in seawater

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Production and transformation of dissolved neutral sugars and amino acids by bacteria in seawater. / Jørgensen, Linda; Lechtenfeld, O.J.; Benner, R.; Middelboe, Mathias; Stedmon, C.A.

In: Biogeosciences, Vol. 11, 2014, p. 5349-5363.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Jørgensen, L, Lechtenfeld, OJ, Benner, R, Middelboe, M & Stedmon, CA 2014, 'Production and transformation of dissolved neutral sugars and amino acids by bacteria in seawater', Biogeosciences, vol. 11, pp. 5349-5363. https://doi.org/10.5194/bg-11-5349-2014

APA

Jørgensen, L., Lechtenfeld, O. J., Benner, R., Middelboe, M., & Stedmon, C. A. (2014). Production and transformation of dissolved neutral sugars and amino acids by bacteria in seawater. Biogeosciences, 11, 5349-5363. https://doi.org/10.5194/bg-11-5349-2014

Vancouver

Jørgensen L, Lechtenfeld OJ, Benner R, Middelboe M, Stedmon CA. Production and transformation of dissolved neutral sugars and amino acids by bacteria in seawater. Biogeosciences. 2014;11:5349-5363. https://doi.org/10.5194/bg-11-5349-2014

Author

Jørgensen, Linda ; Lechtenfeld, O.J. ; Benner, R. ; Middelboe, Mathias ; Stedmon, C.A. / Production and transformation of dissolved neutral sugars and amino acids by bacteria in seawater. In: Biogeosciences. 2014 ; Vol. 11. pp. 5349-5363.

Bibtex

@article{988b8bb78b25436fa25fd79130b9e295,
title = "Production and transformation of dissolved neutral sugars and amino acids by bacteria in seawater",
abstract = "Dissolved organic matter (DOM) in the ocean consists of a heterogeneous mixture of molecules, most of which are of unknown origin. Neutral sugars and amino acids are among the few recognizable biomolecules in DOM, and the molecular composition of these biomolecules is shaped primarily by biological production and degradation processes. This study provides insight into the bioavailability of biomolecules as well as the chemical composition of DOM produced by bacteria. The molecular compositions of combined neutral sugars and amino acids were investigated in DOM produced by bacteria and in DOM remaining after 32 days of bacterial degradation. Results from bioassay incubations with natural seawater (sampled from water masses originating from the surface waters of the Arctic Ocean and the North Atlantic Ocean) and artificial seawater indicate that the molecular compositions following bacterial degradation are not strongly influenced by the initial substrate or bacterial community. The molecular composition of neutral sugars released by bacteria was characterized by a high glucose content (47 mol %) and heterogeneous contributions from other neutral sugars (3-14 mol %). DOM remaining after bacterial degradation was characterized by a high galactose content (33 mol %), followed by glucose (22 mol %) and the remaining neutral sugars (7-11 mol %). The ratio of D-amino acids to L-amino acids increased during the experiments as a response to bacterial degradation, and after 32 days, the D/L ratios of aspartic acid, glutamic acid, serine and alanine reached around 0.79, 0.32, 0.30 and 0.51 in all treatments, respectively. The striking similarity in neutral sugar and amino acid compositions between natural (representing marine semi-labile and refractory DOM) and artificial (representing bacterially produced DOM) seawater samples, suggests that microbes transform bioavailable neutral sugars and amino acids into a common, more persistent form.",
author = "Linda J{\o}rgensen and O.J. Lechtenfeld and R. Benner and Mathias Middelboe and C.A. Stedmon",
year = "2014",
doi = "10.5194/bg-11-5349-2014",
language = "English",
volume = "11",
pages = "5349--5363",
journal = "Biogeosciences",
issn = "1726-4170",
publisher = "Copernicus GmbH",

}

RIS

TY - JOUR

T1 - Production and transformation of dissolved neutral sugars and amino acids by bacteria in seawater

AU - Jørgensen, Linda

AU - Lechtenfeld, O.J.

AU - Benner, R.

AU - Middelboe, Mathias

AU - Stedmon, C.A.

PY - 2014

Y1 - 2014

N2 - Dissolved organic matter (DOM) in the ocean consists of a heterogeneous mixture of molecules, most of which are of unknown origin. Neutral sugars and amino acids are among the few recognizable biomolecules in DOM, and the molecular composition of these biomolecules is shaped primarily by biological production and degradation processes. This study provides insight into the bioavailability of biomolecules as well as the chemical composition of DOM produced by bacteria. The molecular compositions of combined neutral sugars and amino acids were investigated in DOM produced by bacteria and in DOM remaining after 32 days of bacterial degradation. Results from bioassay incubations with natural seawater (sampled from water masses originating from the surface waters of the Arctic Ocean and the North Atlantic Ocean) and artificial seawater indicate that the molecular compositions following bacterial degradation are not strongly influenced by the initial substrate or bacterial community. The molecular composition of neutral sugars released by bacteria was characterized by a high glucose content (47 mol %) and heterogeneous contributions from other neutral sugars (3-14 mol %). DOM remaining after bacterial degradation was characterized by a high galactose content (33 mol %), followed by glucose (22 mol %) and the remaining neutral sugars (7-11 mol %). The ratio of D-amino acids to L-amino acids increased during the experiments as a response to bacterial degradation, and after 32 days, the D/L ratios of aspartic acid, glutamic acid, serine and alanine reached around 0.79, 0.32, 0.30 and 0.51 in all treatments, respectively. The striking similarity in neutral sugar and amino acid compositions between natural (representing marine semi-labile and refractory DOM) and artificial (representing bacterially produced DOM) seawater samples, suggests that microbes transform bioavailable neutral sugars and amino acids into a common, more persistent form.

AB - Dissolved organic matter (DOM) in the ocean consists of a heterogeneous mixture of molecules, most of which are of unknown origin. Neutral sugars and amino acids are among the few recognizable biomolecules in DOM, and the molecular composition of these biomolecules is shaped primarily by biological production and degradation processes. This study provides insight into the bioavailability of biomolecules as well as the chemical composition of DOM produced by bacteria. The molecular compositions of combined neutral sugars and amino acids were investigated in DOM produced by bacteria and in DOM remaining after 32 days of bacterial degradation. Results from bioassay incubations with natural seawater (sampled from water masses originating from the surface waters of the Arctic Ocean and the North Atlantic Ocean) and artificial seawater indicate that the molecular compositions following bacterial degradation are not strongly influenced by the initial substrate or bacterial community. The molecular composition of neutral sugars released by bacteria was characterized by a high glucose content (47 mol %) and heterogeneous contributions from other neutral sugars (3-14 mol %). DOM remaining after bacterial degradation was characterized by a high galactose content (33 mol %), followed by glucose (22 mol %) and the remaining neutral sugars (7-11 mol %). The ratio of D-amino acids to L-amino acids increased during the experiments as a response to bacterial degradation, and after 32 days, the D/L ratios of aspartic acid, glutamic acid, serine and alanine reached around 0.79, 0.32, 0.30 and 0.51 in all treatments, respectively. The striking similarity in neutral sugar and amino acid compositions between natural (representing marine semi-labile and refractory DOM) and artificial (representing bacterially produced DOM) seawater samples, suggests that microbes transform bioavailable neutral sugars and amino acids into a common, more persistent form.

U2 - 10.5194/bg-11-5349-2014

DO - 10.5194/bg-11-5349-2014

M3 - Journal article

AN - SCOPUS:84907927219

VL - 11

SP - 5349

EP - 5363

JO - Biogeosciences

JF - Biogeosciences

SN - 1726-4170

ER -

ID: 128384185