Proteinase production in Pseudomonas fluorescens ON2 is affected by carbon sources and allows surface-attached but not planktonic cells to utilize protein for growth in lake water

Research output: Contribution to journalJournal articleResearchpeer-review

Standard

Proteinase production in Pseudomonas fluorescens ON2 is affected by carbon sources and allows surface-attached but not planktonic cells to utilize protein for growth in lake water. / Nicolaisen, Mette Haubjerg; Worm, Jakob; Jørgensen, Niels O. G.; Middelboe, Mathias; Nybroe, Ole.

In: F E M S Microbiology Ecology, Vol. 80, No. 1, 2012, p. 168-178.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Nicolaisen, MH, Worm, J, Jørgensen, NOG, Middelboe, M & Nybroe, O 2012, 'Proteinase production in Pseudomonas fluorescens ON2 is affected by carbon sources and allows surface-attached but not planktonic cells to utilize protein for growth in lake water', F E M S Microbiology Ecology, vol. 80, no. 1, pp. 168-178. https://doi.org/10.1111/j.1574-6941.2011.01285.x

APA

Nicolaisen, M. H., Worm, J., Jørgensen, N. O. G., Middelboe, M., & Nybroe, O. (2012). Proteinase production in Pseudomonas fluorescens ON2 is affected by carbon sources and allows surface-attached but not planktonic cells to utilize protein for growth in lake water. F E M S Microbiology Ecology, 80(1), 168-178. https://doi.org/10.1111/j.1574-6941.2011.01285.x

Vancouver

Nicolaisen MH, Worm J, Jørgensen NOG, Middelboe M, Nybroe O. Proteinase production in Pseudomonas fluorescens ON2 is affected by carbon sources and allows surface-attached but not planktonic cells to utilize protein for growth in lake water. F E M S Microbiology Ecology. 2012;80(1):168-178. https://doi.org/10.1111/j.1574-6941.2011.01285.x

Author

Nicolaisen, Mette Haubjerg ; Worm, Jakob ; Jørgensen, Niels O. G. ; Middelboe, Mathias ; Nybroe, Ole. / Proteinase production in Pseudomonas fluorescens ON2 is affected by carbon sources and allows surface-attached but not planktonic cells to utilize protein for growth in lake water. In: F E M S Microbiology Ecology. 2012 ; Vol. 80, No. 1. pp. 168-178.

Bibtex

@article{0770478fb4bb43f0bb4b314b78770e6f,
title = "Proteinase production in Pseudomonas fluorescens ON2 is affected by carbon sources and allows surface-attached but not planktonic cells to utilize protein for growth in lake water",
abstract = "Proteins may be an important carbon and nitrogen source to bacteria in aquatic habitats, yet knowledge on the actual utilization of this substrate by proteolytic bacteria is scarce. In the present study, Pseudomonas fluorescens ON2 produced an alkaline proteinase (AprX) during growth and there was no evidence for cell density-regulated or starvation-induced proteinase production. Proteinase was produced in the absence of an organic nitrogen source, and citrate had a negative while glucose had a positive effect on the production. Hence P. fluorescens ON2 seems to exploit protein sources by expressing the proteinase during growth unless a preferred carbon source like citrate is present. Lake water model systems were subsequently used to investigate the ability of proteolytic versus non-proteolytic ON2 strains to utilize protein for growth at moderate cell densities. Only cells forming surface-attached micro-colonies were able to utilize this resource, while planktonic cells were not. Our experiments are the first to experimentally support models predicting that production of extra-cellular enzymes in dilute environments may be a waste of resources, whereas it represents a favorable feeding strategy in organic matrices like detritus, micro-colonies or biofilm.",
author = "Nicolaisen, {Mette Haubjerg} and Jakob Worm and J{\o}rgensen, {Niels O. G.} and Mathias Middelboe and Ole Nybroe",
note = "{\textcopyright} 2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.",
year = "2012",
doi = "10.1111/j.1574-6941.2011.01285.x",
language = "English",
volume = "80",
pages = "168--178",
journal = "F E M S Microbiology Ecology",
issn = "0168-6496",
publisher = "Oxford University Press",
number = "1",

}

RIS

TY - JOUR

T1 - Proteinase production in Pseudomonas fluorescens ON2 is affected by carbon sources and allows surface-attached but not planktonic cells to utilize protein for growth in lake water

AU - Nicolaisen, Mette Haubjerg

AU - Worm, Jakob

AU - Jørgensen, Niels O. G.

AU - Middelboe, Mathias

AU - Nybroe, Ole

N1 - © 2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.

PY - 2012

Y1 - 2012

N2 - Proteins may be an important carbon and nitrogen source to bacteria in aquatic habitats, yet knowledge on the actual utilization of this substrate by proteolytic bacteria is scarce. In the present study, Pseudomonas fluorescens ON2 produced an alkaline proteinase (AprX) during growth and there was no evidence for cell density-regulated or starvation-induced proteinase production. Proteinase was produced in the absence of an organic nitrogen source, and citrate had a negative while glucose had a positive effect on the production. Hence P. fluorescens ON2 seems to exploit protein sources by expressing the proteinase during growth unless a preferred carbon source like citrate is present. Lake water model systems were subsequently used to investigate the ability of proteolytic versus non-proteolytic ON2 strains to utilize protein for growth at moderate cell densities. Only cells forming surface-attached micro-colonies were able to utilize this resource, while planktonic cells were not. Our experiments are the first to experimentally support models predicting that production of extra-cellular enzymes in dilute environments may be a waste of resources, whereas it represents a favorable feeding strategy in organic matrices like detritus, micro-colonies or biofilm.

AB - Proteins may be an important carbon and nitrogen source to bacteria in aquatic habitats, yet knowledge on the actual utilization of this substrate by proteolytic bacteria is scarce. In the present study, Pseudomonas fluorescens ON2 produced an alkaline proteinase (AprX) during growth and there was no evidence for cell density-regulated or starvation-induced proteinase production. Proteinase was produced in the absence of an organic nitrogen source, and citrate had a negative while glucose had a positive effect on the production. Hence P. fluorescens ON2 seems to exploit protein sources by expressing the proteinase during growth unless a preferred carbon source like citrate is present. Lake water model systems were subsequently used to investigate the ability of proteolytic versus non-proteolytic ON2 strains to utilize protein for growth at moderate cell densities. Only cells forming surface-attached micro-colonies were able to utilize this resource, while planktonic cells were not. Our experiments are the first to experimentally support models predicting that production of extra-cellular enzymes in dilute environments may be a waste of resources, whereas it represents a favorable feeding strategy in organic matrices like detritus, micro-colonies or biofilm.

U2 - 10.1111/j.1574-6941.2011.01285.x

DO - 10.1111/j.1574-6941.2011.01285.x

M3 - Journal article

C2 - 22224410

VL - 80

SP - 168

EP - 178

JO - F E M S Microbiology Ecology

JF - F E M S Microbiology Ecology

SN - 0168-6496

IS - 1

ER -

ID: 37555055