Inherent lack of CMFDA/FDA staining in certain algae and its implication for ballast water testing
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Inherent lack of CMFDA/FDA staining in certain algae and its implication for ballast water testing. / Hansen, Gert; Petersen, Gitte; Maguet, Rémi; Sim, Esther; Madsen, Torben.
In: Marine Pollution Bulletin, Vol. 194, No. Part B, 115312, 2023.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Inherent lack of CMFDA/FDA staining in certain algae and its implication for ballast water testing
AU - Hansen, Gert
AU - Petersen, Gitte
AU - Maguet, Rémi
AU - Sim, Esther
AU - Madsen, Torben
N1 - Copyright © 2023 Elsevier Ltd. All rights reserved.
PY - 2023
Y1 - 2023
N2 - The approved method for testing the efficacy of ballast water management systems with respect to killing 10-50 μm organisms uses movements of the organisms or the vital stains CMFDA/FDA. The present study demonstrates that certain freshwater coccoid chlorophytes, known or suspected to contain a highly resistant cell wall component (algaenan), stain poorly with CMFDA/FDA, resulting in false negatives. The staining rates for the most dominant species were determined and were approx. 3-70 %. The use of Crystal Violet as an indicator for the presence of algaenan gave inconclusive results. The number of the 10-50 μm organisms in a small pond was found to be 10,183 organisms/mL (Lugol's fixed sample) vs. 2335 organisms/mL (CMFDA/FDA-stained sample). Using the staining rates obtained, it was estimated that the number of false negatives could make 40-50 %. The implications for biological performance evaluation of ballast water management systems are discussed.
AB - The approved method for testing the efficacy of ballast water management systems with respect to killing 10-50 μm organisms uses movements of the organisms or the vital stains CMFDA/FDA. The present study demonstrates that certain freshwater coccoid chlorophytes, known or suspected to contain a highly resistant cell wall component (algaenan), stain poorly with CMFDA/FDA, resulting in false negatives. The staining rates for the most dominant species were determined and were approx. 3-70 %. The use of Crystal Violet as an indicator for the presence of algaenan gave inconclusive results. The number of the 10-50 μm organisms in a small pond was found to be 10,183 organisms/mL (Lugol's fixed sample) vs. 2335 organisms/mL (CMFDA/FDA-stained sample). Using the staining rates obtained, it was estimated that the number of false negatives could make 40-50 %. The implications for biological performance evaluation of ballast water management systems are discussed.
U2 - 10.1016/j.marpolbul.2023.115312
DO - 10.1016/j.marpolbul.2023.115312
M3 - Journal article
C2 - 37481801
VL - 194
JO - Marine Pollution Bulletin
JF - Marine Pollution Bulletin
SN - 0025-326X
IS - Part B
M1 - 115312
ER -
ID: 360135181