Non-invasive investigation of the morphology and optical properties of the upside-down jellyfish Cassiopea with optical coherence tomography

Research output: Contribution to journal › Journal article › Research › peer-review

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Non-invasive investigation of the morphology and optical properties of the upside-down jellyfish Cassiopea with optical coherence tomography. / Lyndby, Niclas Heidelberg; Murthy, Swathi; Bessette, Sandrine; Jakobsen, Sofie Lindegaard; Meibom, Anders; Kühl, Michael.

In: Proceedings of the Royal Society B: Biological Sciences, Vol. 290, No. 2007, 20230127, 2023.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Lyndby, NH, Murthy, S, Bessette, S, Jakobsen, SL, Meibom, A & Kühl, M 2023, 'Non-invasive investigation of the morphology and optical properties of the upside-down jellyfish Cassiopea with optical coherence tomography', Proceedings of the Royal Society B: Biological Sciences, vol. 290, no. 2007, 20230127. https://doi.org/10.1098/rspb.2023.0127

APA

Lyndby, N. H., Murthy, S., Bessette, S., Jakobsen, S. L., Meibom, A., & Kühl, M. (2023). Non-invasive investigation of the morphology and optical properties of the upside-down jellyfish Cassiopea with optical coherence tomography. Proceedings of the Royal Society B: Biological Sciences, 290(2007), [20230127]. https://doi.org/10.1098/rspb.2023.0127

Vancouver

Lyndby NH, Murthy S, Bessette S, Jakobsen SL, Meibom A, Kühl M. Non-invasive investigation of the morphology and optical properties of the upside-down jellyfish Cassiopea with optical coherence tomography. Proceedings of the Royal Society B: Biological Sciences. 2023;290(2007). 20230127. https://doi.org/10.1098/rspb.2023.0127

Author

Lyndby, Niclas Heidelberg ; Murthy, Swathi ; Bessette, Sandrine ; Jakobsen, Sofie Lindegaard ; Meibom, Anders ; Kühl, Michael. / Non-invasive investigation of the morphology and optical properties of the upside-down jellyfish Cassiopea with optical coherence tomography. In: Proceedings of the Royal Society B: Biological Sciences. 2023 ; Vol. 290, No. 2007.

Bibtex

@article{96b71f83edb94081aa26a74c327d052a,
title = "Non-invasive investigation of the morphology and optical properties of the upside-down jellyfish Cassiopea with optical coherence tomography",
abstract = "The jellyfish Cassiopea largely cover their carbon demand via photosynthates produced by microalgal endosymbionts, but how holobiont morphology and tissue optical properties affect the light microclimate and symbiont photosynthesis in Cassiopea remain unexplored. Here, we use optical coherence tomography (OCT) to study the morphology of Cassiopea medusae at high spatial resolution. We include detailed 3D reconstructions of external micromorphology, and show the spatial distribution of endosymbionts and white granules in the bell tissue. Furthermore, we use OCT data to extract inherent optical properties from light-scattering white granules in Cassiopea, and show that granules enhance local light-availability for symbionts in close proximity. Individual granules had a scattering coefficient of µs = 200-300 cm-1, and scattering anisotropy factor of g = 0.7, while large tissue-regions filled with white granules had a lower µs = 40-100 cm-1, and g = 0.8-0.9. We combined OCT information with isotopic labelling experiments to investigate the effect of enhanced light-availability in whitish tissue regions. Endosymbionts located in whitish tissue exhibited significantly higher carbon fixation compared to symbionts in anastomosing tissue (i.e. tissue without light-scattering white granules). Our findings support previous suggestions that white granules in Cassiopea play an important role in the host modulation of the light-microenvironment.",
keywords = "chlorophyll fluorescence, Cnidaria, NanoSIMS, optical properties, symbiosis",
author = "Lyndby, {Niclas Heidelberg} and Swathi Murthy and Sandrine Bessette and Jakobsen, {Sofie Lindegaard} and Anders Meibom and Michael K{\"u}hl",
year = "2023",
doi = "10.1098/rspb.2023.0127",
language = "English",
volume = "290",
journal = "Proceedings of the Royal Society B: Biological Sciences",
issn = "0962-8452",
publisher = "The Royal Society Publishing",
number = "2007",

}

RIS

TY - JOUR

T1 - Non-invasive investigation of the morphology and optical properties of the upside-down jellyfish Cassiopea with optical coherence tomography

AU - Lyndby, Niclas Heidelberg

AU - Murthy, Swathi

AU - Bessette, Sandrine

AU - Jakobsen, Sofie Lindegaard

AU - Meibom, Anders

AU - Kühl, Michael

PY - 2023

Y1 - 2023

N2 - The jellyfish Cassiopea largely cover their carbon demand via photosynthates produced by microalgal endosymbionts, but how holobiont morphology and tissue optical properties affect the light microclimate and symbiont photosynthesis in Cassiopea remain unexplored. Here, we use optical coherence tomography (OCT) to study the morphology of Cassiopea medusae at high spatial resolution. We include detailed 3D reconstructions of external micromorphology, and show the spatial distribution of endosymbionts and white granules in the bell tissue. Furthermore, we use OCT data to extract inherent optical properties from light-scattering white granules in Cassiopea, and show that granules enhance local light-availability for symbionts in close proximity. Individual granules had a scattering coefficient of µs = 200-300 cm-1, and scattering anisotropy factor of g = 0.7, while large tissue-regions filled with white granules had a lower µs = 40-100 cm-1, and g = 0.8-0.9. We combined OCT information with isotopic labelling experiments to investigate the effect of enhanced light-availability in whitish tissue regions. Endosymbionts located in whitish tissue exhibited significantly higher carbon fixation compared to symbionts in anastomosing tissue (i.e. tissue without light-scattering white granules). Our findings support previous suggestions that white granules in Cassiopea play an important role in the host modulation of the light-microenvironment.

AB - The jellyfish Cassiopea largely cover their carbon demand via photosynthates produced by microalgal endosymbionts, but how holobiont morphology and tissue optical properties affect the light microclimate and symbiont photosynthesis in Cassiopea remain unexplored. Here, we use optical coherence tomography (OCT) to study the morphology of Cassiopea medusae at high spatial resolution. We include detailed 3D reconstructions of external micromorphology, and show the spatial distribution of endosymbionts and white granules in the bell tissue. Furthermore, we use OCT data to extract inherent optical properties from light-scattering white granules in Cassiopea, and show that granules enhance local light-availability for symbionts in close proximity. Individual granules had a scattering coefficient of µs = 200-300 cm-1, and scattering anisotropy factor of g = 0.7, while large tissue-regions filled with white granules had a lower µs = 40-100 cm-1, and g = 0.8-0.9. We combined OCT information with isotopic labelling experiments to investigate the effect of enhanced light-availability in whitish tissue regions. Endosymbionts located in whitish tissue exhibited significantly higher carbon fixation compared to symbionts in anastomosing tissue (i.e. tissue without light-scattering white granules). Our findings support previous suggestions that white granules in Cassiopea play an important role in the host modulation of the light-microenvironment.

KW - chlorophyll fluorescence

KW - Cnidaria

KW - NanoSIMS

KW - optical properties

KW - symbiosis

U2 - 10.1098/rspb.2023.0127

DO - 10.1098/rspb.2023.0127

M3 - Journal article

C2 - 37752841

AN - SCOPUS:85172673846

VL - 290

JO - Proceedings of the Royal Society B: Biological Sciences

JF - Proceedings of the Royal Society B: Biological Sciences

SN - 0962-8452

IS - 2007

M1 - 20230127

ER -

ID: 369981787