Simultaneous visualization of flow fields and oxygen concentrations to unravel transport and metabolic processes in biological systems

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Simultaneous visualization of flow fields and oxygen concentrations to unravel transport and metabolic processes in biological systems. / Ahmerkamp, Soeren; Jalaluddin, Farooq Moin; Cui, Yuan; Brumley, Douglas R.; Pacherres, Cesar O.; Berg, Jasmine S.; Stocker, Roman; Kuypers, Marcel M. M.; Koren, Klaus; Behrendt, Lars.

In: Cell Reports Methods, Vol. 2, No. 5, 100216, 2022.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Ahmerkamp, S, Jalaluddin, FM, Cui, Y, Brumley, DR, Pacherres, CO, Berg, JS, Stocker, R, Kuypers, MMM, Koren, K & Behrendt, L 2022, 'Simultaneous visualization of flow fields and oxygen concentrations to unravel transport and metabolic processes in biological systems', Cell Reports Methods, vol. 2, no. 5, 100216. https://doi.org/10.1016/j.crmeth.2022.100216

APA

Ahmerkamp, S., Jalaluddin, F. M., Cui, Y., Brumley, D. R., Pacherres, C. O., Berg, J. S., Stocker, R., Kuypers, M. M. M., Koren, K., & Behrendt, L. (2022). Simultaneous visualization of flow fields and oxygen concentrations to unravel transport and metabolic processes in biological systems. Cell Reports Methods, 2(5), [100216]. https://doi.org/10.1016/j.crmeth.2022.100216

Vancouver

Ahmerkamp S, Jalaluddin FM, Cui Y, Brumley DR, Pacherres CO, Berg JS et al. Simultaneous visualization of flow fields and oxygen concentrations to unravel transport and metabolic processes in biological systems. Cell Reports Methods. 2022;2(5). 100216. https://doi.org/10.1016/j.crmeth.2022.100216

Author

Ahmerkamp, Soeren ; Jalaluddin, Farooq Moin ; Cui, Yuan ; Brumley, Douglas R. ; Pacherres, Cesar O. ; Berg, Jasmine S. ; Stocker, Roman ; Kuypers, Marcel M. M. ; Koren, Klaus ; Behrendt, Lars. / Simultaneous visualization of flow fields and oxygen concentrations to unravel transport and metabolic processes in biological systems. In: Cell Reports Methods. 2022 ; Vol. 2, No. 5.

Bibtex

@article{f81677f6bfde4b049d3fac357dba22be,
title = "Simultaneous visualization of flow fields and oxygen concentrations to unravel transport and metabolic processes in biological systems",
abstract = "From individual cells to whole organisms, O-2 transport unfolds across micrometer- tomillimeter-length scales and can change within milliseconds in response to fluid flows and organismal behavior. The spatiotemporal complexity of these processes makes the accurate assessment of O-2 dynamics via currently availablemethods difficult or unreliable. Here, we present {"}sensPIV,'' a method to simultaneously measure O-2 concentrations and flow fields. By tracking O-2-sensitive microparticles in flow using imaging technologies that allow for instantaneous referencing, wemeasuredO(2) transportwithin (1) microfluidic devices, (2) sinkingmodel aggregates, and (3) complex colony-forming corals. Through the use of sensPIV, we find that corals use ciliarymovement to link zones of photosynthetic O-2 production to zones of O-2 consumption. SensPIV can potentially be extendable to study flow-organism interactions across many life-science and engineering applications.",
keywords = "PARTICLE IMAGE VELOCIMETRY, O-2 DYNAMICS, FLUID, RHIZOSPHERE, VERSATILE, SEDIMENTS, FLUXES, ROOTS, CELLS, LIVE",
author = "Soeren Ahmerkamp and Jalaluddin, {Farooq Moin} and Yuan Cui and Brumley, {Douglas R.} and Pacherres, {Cesar O.} and Berg, {Jasmine S.} and Roman Stocker and Kuypers, {Marcel M. M.} and Klaus Koren and Lars Behrendt",
year = "2022",
doi = "10.1016/j.crmeth.2022.100216",
language = "English",
volume = "2",
journal = "Cell Reports Methods",
issn = "2667-2375",
publisher = "Cell Press",
number = "5",

}

RIS

TY - JOUR

T1 - Simultaneous visualization of flow fields and oxygen concentrations to unravel transport and metabolic processes in biological systems

AU - Ahmerkamp, Soeren

AU - Jalaluddin, Farooq Moin

AU - Cui, Yuan

AU - Brumley, Douglas R.

AU - Pacherres, Cesar O.

AU - Berg, Jasmine S.

AU - Stocker, Roman

AU - Kuypers, Marcel M. M.

AU - Koren, Klaus

AU - Behrendt, Lars

PY - 2022

Y1 - 2022

N2 - From individual cells to whole organisms, O-2 transport unfolds across micrometer- tomillimeter-length scales and can change within milliseconds in response to fluid flows and organismal behavior. The spatiotemporal complexity of these processes makes the accurate assessment of O-2 dynamics via currently availablemethods difficult or unreliable. Here, we present "sensPIV,'' a method to simultaneously measure O-2 concentrations and flow fields. By tracking O-2-sensitive microparticles in flow using imaging technologies that allow for instantaneous referencing, wemeasuredO(2) transportwithin (1) microfluidic devices, (2) sinkingmodel aggregates, and (3) complex colony-forming corals. Through the use of sensPIV, we find that corals use ciliarymovement to link zones of photosynthetic O-2 production to zones of O-2 consumption. SensPIV can potentially be extendable to study flow-organism interactions across many life-science and engineering applications.

AB - From individual cells to whole organisms, O-2 transport unfolds across micrometer- tomillimeter-length scales and can change within milliseconds in response to fluid flows and organismal behavior. The spatiotemporal complexity of these processes makes the accurate assessment of O-2 dynamics via currently availablemethods difficult or unreliable. Here, we present "sensPIV,'' a method to simultaneously measure O-2 concentrations and flow fields. By tracking O-2-sensitive microparticles in flow using imaging technologies that allow for instantaneous referencing, wemeasuredO(2) transportwithin (1) microfluidic devices, (2) sinkingmodel aggregates, and (3) complex colony-forming corals. Through the use of sensPIV, we find that corals use ciliarymovement to link zones of photosynthetic O-2 production to zones of O-2 consumption. SensPIV can potentially be extendable to study flow-organism interactions across many life-science and engineering applications.

KW - PARTICLE IMAGE VELOCIMETRY

KW - O-2 DYNAMICS

KW - FLUID

KW - RHIZOSPHERE

KW - VERSATILE

KW - SEDIMENTS

KW - FLUXES

KW - ROOTS

KW - CELLS

KW - LIVE

U2 - 10.1016/j.crmeth.2022.100216

DO - 10.1016/j.crmeth.2022.100216

M3 - Journal article

C2 - 35637907

VL - 2

JO - Cell Reports Methods

JF - Cell Reports Methods

SN - 2667-2375

IS - 5

M1 - 100216

ER -

ID: 335675957