The Na+/H+ exchanger NHE1 is required for directional migration stimulated via PDGFR-alpha in the primary cilium

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

The Na+/H+ exchanger NHE1 is required for directional migration stimulated via PDGFR-alpha in the primary cilium. / Schneider, Linda; Stock, Christian-Martin; Dieterich, Peter; Jensen, Bo Hammer; Pedersen, Lotte Bang; Satir, Peter; Schwab, Albrecht; Christensen, Søren Tvorup; Pedersen, Stine Falsig.

In: Journal of Cell Biology, Vol. 185, No. 1, 2009, p. 163-76.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Schneider, L, Stock, C-M, Dieterich, P, Jensen, BH, Pedersen, LB, Satir, P, Schwab, A, Christensen, ST & Pedersen, SF 2009, 'The Na+/H+ exchanger NHE1 is required for directional migration stimulated via PDGFR-alpha in the primary cilium', Journal of Cell Biology, vol. 185, no. 1, pp. 163-76. https://doi.org/10.1083/jcb.200806019

APA

Schneider, L., Stock, C-M., Dieterich, P., Jensen, B. H., Pedersen, L. B., Satir, P., Schwab, A., Christensen, S. T., & Pedersen, S. F. (2009). The Na+/H+ exchanger NHE1 is required for directional migration stimulated via PDGFR-alpha in the primary cilium. Journal of Cell Biology, 185(1), 163-76. https://doi.org/10.1083/jcb.200806019

Vancouver

Schneider L, Stock C-M, Dieterich P, Jensen BH, Pedersen LB, Satir P et al. The Na+/H+ exchanger NHE1 is required for directional migration stimulated via PDGFR-alpha in the primary cilium. Journal of Cell Biology. 2009;185(1):163-76. https://doi.org/10.1083/jcb.200806019

Author

Schneider, Linda ; Stock, Christian-Martin ; Dieterich, Peter ; Jensen, Bo Hammer ; Pedersen, Lotte Bang ; Satir, Peter ; Schwab, Albrecht ; Christensen, Søren Tvorup ; Pedersen, Stine Falsig. / The Na+/H+ exchanger NHE1 is required for directional migration stimulated via PDGFR-alpha in the primary cilium. In: Journal of Cell Biology. 2009 ; Vol. 185, No. 1. pp. 163-76.

Bibtex

@article{4fdb7fb025f311df8ed1000ea68e967b,
title = "The Na+/H+ exchanger NHE1 is required for directional migration stimulated via PDGFR-alpha in the primary cilium",
abstract = "We previously demonstrated that the primary cilium coordinates platelet-derived growth factor (PDGF) receptor (PDGFR) alpha-mediated migration in growth-arrested fibroblasts. In this study, we investigate the functional relationship between ciliary PDGFR-alpha and the Na(+)/H(+) exchanger NHE1 in directional cell migration. NHE1 messenger RNA and protein levels are up-regulated in NIH3T3 cells and mouse embryonic fibroblasts (MEFs) during growth arrest, which is concomitant with cilium formation. NHE1 up-regulation is unaffected in Tg737(orpk) MEFs, which have no or very short primary cilia. In growth-arrested NIH3T3 cells, NHE1 is activated by the specific PDGFR-alpha ligand PDGF-AA. In wound-healing assays on growth-arrested NIH3T3 cells and wild-type MEFs, NHE1 inhibition by 5'-(N-ethyl-N-isopropyl) amiloride potently reduces PDGF-AA-mediated directional migration. These effects are strongly attenuated in interphase NIH3T3 cells, which are devoid of primary cilia, and in Tg737(orpk) MEFs. PDGF-AA failed to stimulate migration in NHE1-null fibroblasts. In conclusion, stimulation of directional migration in response to ciliary PDGFR-alpha signals is specifically dependent on NHE1 activity, indicating that NHE1 activation is a critical event in the physiological response to PDGFR-alpha stimulation.",
author = "Linda Schneider and Christian-Martin Stock and Peter Dieterich and Jensen, {Bo Hammer} and Pedersen, {Lotte Bang} and Peter Satir and Albrecht Schwab and Christensen, {S{\o}ren Tvorup} and Pedersen, {Stine Falsig}",
note = "Keywords: Amiloride; Animals; Cation Transport Proteins; Cell Line; Cell Movement; Cilia; Interphase; Mice; NIH 3T3 Cells; Platelet-Derived Growth Factor; RNA, Messenger; Receptor, Platelet-Derived Growth Factor alpha; Signal Transduction; Sodium-Hydrogen Antiporter; Up-Regulation",
year = "2009",
doi = "10.1083/jcb.200806019",
language = "English",
volume = "185",
pages = "163--76",
journal = "Journal of Cell Biology",
issn = "0021-9525",
publisher = "Rockefeller University Press",
number = "1",

}

RIS

TY - JOUR

T1 - The Na+/H+ exchanger NHE1 is required for directional migration stimulated via PDGFR-alpha in the primary cilium

AU - Schneider, Linda

AU - Stock, Christian-Martin

AU - Dieterich, Peter

AU - Jensen, Bo Hammer

AU - Pedersen, Lotte Bang

AU - Satir, Peter

AU - Schwab, Albrecht

AU - Christensen, Søren Tvorup

AU - Pedersen, Stine Falsig

N1 - Keywords: Amiloride; Animals; Cation Transport Proteins; Cell Line; Cell Movement; Cilia; Interphase; Mice; NIH 3T3 Cells; Platelet-Derived Growth Factor; RNA, Messenger; Receptor, Platelet-Derived Growth Factor alpha; Signal Transduction; Sodium-Hydrogen Antiporter; Up-Regulation

PY - 2009

Y1 - 2009

N2 - We previously demonstrated that the primary cilium coordinates platelet-derived growth factor (PDGF) receptor (PDGFR) alpha-mediated migration in growth-arrested fibroblasts. In this study, we investigate the functional relationship between ciliary PDGFR-alpha and the Na(+)/H(+) exchanger NHE1 in directional cell migration. NHE1 messenger RNA and protein levels are up-regulated in NIH3T3 cells and mouse embryonic fibroblasts (MEFs) during growth arrest, which is concomitant with cilium formation. NHE1 up-regulation is unaffected in Tg737(orpk) MEFs, which have no or very short primary cilia. In growth-arrested NIH3T3 cells, NHE1 is activated by the specific PDGFR-alpha ligand PDGF-AA. In wound-healing assays on growth-arrested NIH3T3 cells and wild-type MEFs, NHE1 inhibition by 5'-(N-ethyl-N-isopropyl) amiloride potently reduces PDGF-AA-mediated directional migration. These effects are strongly attenuated in interphase NIH3T3 cells, which are devoid of primary cilia, and in Tg737(orpk) MEFs. PDGF-AA failed to stimulate migration in NHE1-null fibroblasts. In conclusion, stimulation of directional migration in response to ciliary PDGFR-alpha signals is specifically dependent on NHE1 activity, indicating that NHE1 activation is a critical event in the physiological response to PDGFR-alpha stimulation.

AB - We previously demonstrated that the primary cilium coordinates platelet-derived growth factor (PDGF) receptor (PDGFR) alpha-mediated migration in growth-arrested fibroblasts. In this study, we investigate the functional relationship between ciliary PDGFR-alpha and the Na(+)/H(+) exchanger NHE1 in directional cell migration. NHE1 messenger RNA and protein levels are up-regulated in NIH3T3 cells and mouse embryonic fibroblasts (MEFs) during growth arrest, which is concomitant with cilium formation. NHE1 up-regulation is unaffected in Tg737(orpk) MEFs, which have no or very short primary cilia. In growth-arrested NIH3T3 cells, NHE1 is activated by the specific PDGFR-alpha ligand PDGF-AA. In wound-healing assays on growth-arrested NIH3T3 cells and wild-type MEFs, NHE1 inhibition by 5'-(N-ethyl-N-isopropyl) amiloride potently reduces PDGF-AA-mediated directional migration. These effects are strongly attenuated in interphase NIH3T3 cells, which are devoid of primary cilia, and in Tg737(orpk) MEFs. PDGF-AA failed to stimulate migration in NHE1-null fibroblasts. In conclusion, stimulation of directional migration in response to ciliary PDGFR-alpha signals is specifically dependent on NHE1 activity, indicating that NHE1 activation is a critical event in the physiological response to PDGFR-alpha stimulation.

U2 - 10.1083/jcb.200806019

DO - 10.1083/jcb.200806019

M3 - Journal article

C2 - 19349585

VL - 185

SP - 163

EP - 176

JO - Journal of Cell Biology

JF - Journal of Cell Biology

SN - 0021-9525

IS - 1

ER -

ID: 18337925