Isolation of cancer stem cells from squamous cell carcinoma
Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
Different cancer stem cell (CSC) populations can be found in many types of cancer, including squamous cell carcinoma (SSC). Diverse reports showed that CSC play a crucial role in the relapse of different types of cancer. CSC sustains tumor growth due to their capacity to self-renew and their potential to initiate secondary tumors with metastatic cancer features. Therefore, the development of methods for the isolation of CSC is a key step to explore the mechanisms underlying CSC maintenance. In this chapter, we provide a method for isolating CSC from cutaneous SSC using immunofluorescence labeling to allow the specific purification of CSC by fluorescence-activated cell sorting (FACS). This method is based on the use of CSC membrane markers, allowing as well the isolation CSC from different mouse strains.
Original language | English |
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Title of host publication | Skin Stem Cells : Methods and Protocols |
Editors | Kursad Turksen |
Number of pages | 8 |
Publisher | Humana Press |
Publication date | 2019 |
Edition | 2. ed. |
Pages | 407-414 |
ISBN (Print) | 978-1-4939-8869-3 |
ISBN (Electronic) | 978-1-4939-8870-9 |
DOIs | |
Publication status | Published - 2019 |
Series | Methods in Molecular Biology |
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Volume | 1879 |
ISSN | 1064-3745 |
- Cancer stem cells, FACS, Mouse models, Skin, Squamous cell carcinoma
Research areas
ID: 215920873