BIND&MODIFY: a long-range method for single-molecule mapping of chromatin modifications in eukaryotes

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

BIND&MODIFY : a long-range method for single-molecule mapping of chromatin modifications in eukaryotes. / Weng, Zhe; Ruan, Fengying; Chen, Weitian; Chen, Zhichao; Xie, Yeming; Luo, Meng; Xie, Zhe; Zhang, Chen; Wang, Juan; Sun, Yuxin; Fang, Yitong; Guo, Mei; Tan, Chen; Chen, Wenfang; Tong, Yiqin; Li, Yaning; Wang, Hongqi; Tang, Chong.

In: Genome Biology, Vol. 24, No. 1, 61, 2023.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Weng, Z, Ruan, F, Chen, W, Chen, Z, Xie, Y, Luo, M, Xie, Z, Zhang, C, Wang, J, Sun, Y, Fang, Y, Guo, M, Tan, C, Chen, W, Tong, Y, Li, Y, Wang, H & Tang, C 2023, 'BIND&MODIFY: a long-range method for single-molecule mapping of chromatin modifications in eukaryotes', Genome Biology, vol. 24, no. 1, 61. https://doi.org/10.1186/s13059-023-02896-y

APA

Weng, Z., Ruan, F., Chen, W., Chen, Z., Xie, Y., Luo, M., Xie, Z., Zhang, C., Wang, J., Sun, Y., Fang, Y., Guo, M., Tan, C., Chen, W., Tong, Y., Li, Y., Wang, H., & Tang, C. (2023). BIND&MODIFY: a long-range method for single-molecule mapping of chromatin modifications in eukaryotes. Genome Biology, 24(1), [61]. https://doi.org/10.1186/s13059-023-02896-y

Vancouver

Weng Z, Ruan F, Chen W, Chen Z, Xie Y, Luo M et al. BIND&MODIFY: a long-range method for single-molecule mapping of chromatin modifications in eukaryotes. Genome Biology. 2023;24(1). 61. https://doi.org/10.1186/s13059-023-02896-y

Author

Weng, Zhe ; Ruan, Fengying ; Chen, Weitian ; Chen, Zhichao ; Xie, Yeming ; Luo, Meng ; Xie, Zhe ; Zhang, Chen ; Wang, Juan ; Sun, Yuxin ; Fang, Yitong ; Guo, Mei ; Tan, Chen ; Chen, Wenfang ; Tong, Yiqin ; Li, Yaning ; Wang, Hongqi ; Tang, Chong. / BIND&MODIFY : a long-range method for single-molecule mapping of chromatin modifications in eukaryotes. In: Genome Biology. 2023 ; Vol. 24, No. 1.

Bibtex

@article{1a9dae7c442c4007a19c4793fad86b26,
title = "BIND&MODIFY: a long-range method for single-molecule mapping of chromatin modifications in eukaryotes",
abstract = "Epigenetic modifications of histones are associated with development and pathogenesis of disease. Existing approaches cannot provide insights into long-range interactions and represent the average chromatin state. Here we describe BIND&MODIFY, a method using long-read sequencing for profiling histone modifications and transcription factors on individual DNA fibers. We use recombinant fused protein A-M.EcoGII to tether methyltransferase M.EcoGII to protein binding sites to label neighboring regions by methylation. Aggregated BIND&MODIFY signal matches bulk ChIP-seq and CUT&TAG. BIND&MODIFY can simultaneously measure histone modification status, transcription factor binding, and CpG 5mC methylation at single-molecule resolution and also quantifies correlation between local and distal elements.",
keywords = "CpG methylation, CTCF, Epigenetics, H3K27me3, Histone modification, mA, Methyltransferase",
author = "Zhe Weng and Fengying Ruan and Weitian Chen and Zhichao Chen and Yeming Xie and Meng Luo and Zhe Xie and Chen Zhang and Juan Wang and Yuxin Sun and Yitong Fang and Mei Guo and Chen Tan and Wenfang Chen and Yiqin Tong and Yaning Li and Hongqi Wang and Chong Tang",
note = "Publisher Copyright: {\textcopyright} 2023, The Author(s).",
year = "2023",
doi = "10.1186/s13059-023-02896-y",
language = "English",
volume = "24",
journal = "Genome Biology (Online Edition)",
issn = "1474-7596",
publisher = "BioMed Central Ltd.",
number = "1",

}

RIS

TY - JOUR

T1 - BIND&MODIFY

T2 - a long-range method for single-molecule mapping of chromatin modifications in eukaryotes

AU - Weng, Zhe

AU - Ruan, Fengying

AU - Chen, Weitian

AU - Chen, Zhichao

AU - Xie, Yeming

AU - Luo, Meng

AU - Xie, Zhe

AU - Zhang, Chen

AU - Wang, Juan

AU - Sun, Yuxin

AU - Fang, Yitong

AU - Guo, Mei

AU - Tan, Chen

AU - Chen, Wenfang

AU - Tong, Yiqin

AU - Li, Yaning

AU - Wang, Hongqi

AU - Tang, Chong

N1 - Publisher Copyright: © 2023, The Author(s).

PY - 2023

Y1 - 2023

N2 - Epigenetic modifications of histones are associated with development and pathogenesis of disease. Existing approaches cannot provide insights into long-range interactions and represent the average chromatin state. Here we describe BIND&MODIFY, a method using long-read sequencing for profiling histone modifications and transcription factors on individual DNA fibers. We use recombinant fused protein A-M.EcoGII to tether methyltransferase M.EcoGII to protein binding sites to label neighboring regions by methylation. Aggregated BIND&MODIFY signal matches bulk ChIP-seq and CUT&TAG. BIND&MODIFY can simultaneously measure histone modification status, transcription factor binding, and CpG 5mC methylation at single-molecule resolution and also quantifies correlation between local and distal elements.

AB - Epigenetic modifications of histones are associated with development and pathogenesis of disease. Existing approaches cannot provide insights into long-range interactions and represent the average chromatin state. Here we describe BIND&MODIFY, a method using long-read sequencing for profiling histone modifications and transcription factors on individual DNA fibers. We use recombinant fused protein A-M.EcoGII to tether methyltransferase M.EcoGII to protein binding sites to label neighboring regions by methylation. Aggregated BIND&MODIFY signal matches bulk ChIP-seq and CUT&TAG. BIND&MODIFY can simultaneously measure histone modification status, transcription factor binding, and CpG 5mC methylation at single-molecule resolution and also quantifies correlation between local and distal elements.

KW - CpG methylation

KW - CTCF

KW - Epigenetics

KW - H3K27me3

KW - Histone modification

KW - mA

KW - Methyltransferase

U2 - 10.1186/s13059-023-02896-y

DO - 10.1186/s13059-023-02896-y

M3 - Journal article

C2 - 36991510

AN - SCOPUS:85151173500

VL - 24

JO - Genome Biology (Online Edition)

JF - Genome Biology (Online Edition)

SN - 1474-7596

IS - 1

M1 - 61

ER -

ID: 341880699