BIND&MODIFY: a long-range method for single-molecule mapping of chromatin modifications in eukaryotes
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Epigenetic modifications of histones are associated with development and pathogenesis of disease. Existing approaches cannot provide insights into long-range interactions and represent the average chromatin state. Here we describe BIND&MODIFY, a method using long-read sequencing for profiling histone modifications and transcription factors on individual DNA fibers. We use recombinant fused protein A-M.EcoGII to tether methyltransferase M.EcoGII to protein binding sites to label neighboring regions by methylation. Aggregated BIND&MODIFY signal matches bulk ChIP-seq and CUT&TAG. BIND&MODIFY can simultaneously measure histone modification status, transcription factor binding, and CpG 5mC methylation at single-molecule resolution and also quantifies correlation between local and distal elements.
Original language | English |
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Article number | 61 |
Journal | Genome Biology |
Volume | 24 |
Issue number | 1 |
Number of pages | 29 |
ISSN | 1474-7596 |
DOIs | |
Publication status | Published - 2023 |
Bibliographical note
Publisher Copyright:
© 2023, The Author(s).
- CpG methylation, CTCF, Epigenetics, H3K27me3, Histone modification, mA, Methyltransferase
Research areas
ID: 341880699