SBiNLab: Structural Biology and NMR Laboratory
The research and teaching programs of the laboratory are focussed on the structural biology of proteins. Structural Biology of proteins is a discipline of science aiming towards understanding the correlations between molecular structure and function of proteins, which are the vital engines of the biological cell factory.
A wide selection of techniques are implemented at the laboratory including NMR spectroscopy, optical spectroscopy, protein engineering, bioinformatics and molecular biology.
|Birthe B. Kragelund, Professor
KU profile page | Personal web site
By application of NMR spectroscopy, x-ray crystallography, protein engineering and biophysical techniques we seek to map mechanisms of interactions involved in i.e. receptor activation, cell division, signal transduction, immune activation and protein degradation.
|Henriette E. Autzen, Associate Professor
KU profile page | Personal research page
I investigate the molecular mechanisms underlying taste reception by applying cryo electron microscopy (cryo-EM) and complementary biophysicala and biochemical techniques. Complementary to my research in ionchannels and receptors, I develop tools to aid the isolation of membrane proteins and their membranes which is the primary bottle neck in the biochemistry of membrane proteins.
|Kaare Teilum, Associate Professor
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Slow conformational changes are of key importance to enzyme function. We use NMR spectroscopy, X-ray diffraction and computational methods to characterize, interpret and rationalize the effects of point mutations on the flexibility, stability and catalysis of enzymes in general.
|Kresten Lindorff-Larsen, Professor
KU profile page | Personal web site
The primary tools used in our research are computational methods that we use to study the structure and dynamics of proteins. A particular focus is methods that combine experimental data from NMR spectroscopy with molecular simulations. In one set of studies we combine experiments and simulations to provide a detailed structural interpretation of the experimental data. In another methods to predict what mutations are likely to occur and give rise to drug resistance and what possible mutations giving rise to drug resistance would actually render the enzyme inactive.
We offer a range of different PhD projects, often in collaboration with international academic coworkers or industrial domestic partners. As a phd student you are accepted into the PhdDschool at the Faculty of Science and become part of the PhD program in Protein Science. Please apply directly with the individual principal investigators.
We are also organizing PhD Summer Schools in Protein Science at regular intervals offering lectures by prominent international speakers and seminars.
MSc and BSc Student Projects
The group has an extensive collaboration around techniques and subjects. When working in the group you will be confronted with the following techniques either directly in your own project or through the person on the bench next to you. The major techniques used include:
- Protein expression and purification
- Biophysical techniques (protein stability and folding)
- Molecular biology (cloning, PCR, mutagenesis)
- Analytical techniques (UV-VIS and fluorescence spectroscopy, RP-HPLC)
- Molecular dynamics (MD) simulations
- Computational methods integrating MD simulations with data from experiments
- Nuclear Magnetic Resonance Spectroscopy (NMR)
The laboratory houses the departmental NMR facility equipped with state of the art equipment including three NMR magnets operating at 600, 750 and 800 MHz set up for protein NMR.
SBiNLab has a strong focus on education and welcome new students in the lab. We generally have about 8 masters and 8 bachelor students in our laboratories. All students participate in the weekly SBiNLab seminars, where they give at least one short presentation while working in the lab. If you want to hear more about working in the SBiNLab from a student perspective you are welcome to contact one of the students in the staff list.
SBiNLab offers teaching in the B.Sc. and M.Sc. and Ph.D. courses on protein and cell-biology related subjects. You can read more about the courses taught by the members of the section in the list of courses taught by the Section for Biomolecular Sciences.
In SBiNLab we have a large number of projects. Find examples of our projects below.
SBiNLab has state-of-the-art laboratories equipped for liquid protein NMR spectroscopy which is accessible to researchers from all fields via the cOpenNMR facility (https://www1.bio.ku.dk/copennmr). Additionally we use a wide range of other biophysical and biochemical methods. If you are interested in accessing the equipment or have samples for analysis you are welcome on either a pay-for-service, pay-for-access or collaborative basis. Please contact the manager of the cOpenNMR facility (email@example.com) or the section head Kaare Teilum (firstname.lastname@example.org) for inquiries.
Services of particular relevance to industrial or academic collaborators:
- 600, 750 and 2x800 MHz NMR spectrometers equipped for liquid protein spectroscopy
- Protein expression facilities
- JASCO J-810 CD spectrometer
- Bruker MALDI-TOF MS spectrometer
- Zetasizer SLS and DLS instrument
As part of the research in SBiNLab a number of small applications have been developed. Please find the programs in the list below (click on the titles to access the scripts).
The script automatically calculates the sequence-corrected random coil chemical shifts of a protein, while taking the effects of pH and temperature into consideration.
IDDomainSpotter is a sequence-based approach to assess and visualize domain organisation in long intrinsically disordered proteins based on compositional biases.
We are always happy to receive applications from potential postdocs, PhD, Masters, and Bachelor students, and we welcome applications from both Denmark and abroad. If you are interested in joining the lab or just want to know more about our work, find the relevant scientific staff member and contact us by phone or email – or stop by one of the labs in the Biocenter (see map here).
Open positions are advertised broadly in the relevant media.
In addition, chances of obtaining postdoc grants are quite good when applying with Danish and/or international foundations. If you are interested in applying contact the scientific staff member that you would like to work.
The University of Copenhagen offers studentships for students holding a Danish or foreign Masters degree. In addition, possibilities exist to apply for funding from private foundations. Phd students are accepted into the PhD school at the Faculty of Science and become part of the PhD program in Protein Science.
Masters and Bachelor students projects
We always offer both Masters and Bachelor projects within our different areas of research. For more information and a list of current projects see here.
For all types of positions, please contact the staff to discuss specific projects.